Study on regulation machinery of biosynthetic gene expression in filamentous fungi
| Project/Area Number |
17K15467
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Natural medicines
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| Research Institution | Nagoya City University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 植物内生糸状菌 / Paraboeremia sp. Lsl3 / huperzine A / 生産メカニズム / Lycopodiumアルカロイド / 生合成調節機構 / ゲノム解析 / 生合成遺伝子 / 有用二次代謝産物 / 紫外線照射 / Paraboeremia sp. / Lycopodium alkaloids / 12-epi-lycopodine / 二次代謝調節メカニズム / リコポジウムアルカロイド |
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| Outline of Final Research Achievements |
An ultraviolet-irradiated strain of Paraboeremia sp. Lsl3, a Lycopodiaceae plant-derived endophytic fungus was successfully prepared, and the potential production ability of huperzine A, a candidate natural product of Alzheimer’s disease treatment, has been proved. Furthermore, a facile gene manipulation system of Lsl3 strain was established, and the whole genome sequence has been completed.
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| Academic Significance and Societal Importance of the Research Achievements |
アルツハイマー病改善薬候補であるhuperzine Aは、植物により生産される有効成分であることで知られるが、その生産メカニズムはいまだ明らかにされていない。本研究では、新種の植物内生糸状菌がhuperzine Aを潜在的に生産する能力を持つことを証明し、同菌の遺伝子実験システムを整備することで、生合成機構の解明に向けて大きく前進することができた。このことは、huperzine Aの大量生産システム構築への足がかりにもなり、医薬品開発における新しい供給戦略となることが期待される。
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] Phlenumdines D and E, new Lycopodium alkaloids from Phlegmariurus nummulariifolius, and their regulatory effects on macrophage differentiation during tumor development2018
Author(s)
Nakayama W, Fujiwara Y, Kosuge Y, Monthakantirat O, Fujikawa K, Watthana S, Kitanaka S, Makino T, Ishiuchia K.
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Journal Title
Phytochemistry Letters
Volume: 29
Pages: 98-103
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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