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Understanding of pathology intractable acne vulgaris based on microbiome analysis

Research Project

Project/Area Number 17K15524
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Medical pharmacy
Research InstitutionMeiji Pharmaceutical University

Principal Investigator

Cho Otomi  明治薬科大学, 薬学部, 特任研究員 (50573466)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords尋常性ざ瘡 / マイクロバイオーム / 尋常性ざそう / マラセチア / 薬学 / 免疫学
Outline of Final Research Achievements

Microbiome in pus is divided into three types, 1) P. acnes-type, 2) Diversity-type, and 3) Malassezia-type. The strains obtained from the patients with diversity-type showed low sensitive to the standard treatments for acne vulgaris, clindamycin, doxycycline, and minocycline, whereas Malassezia was sensitive to ketoconazole and miconazole. It was considered that the diversity-type would be intractable. When multiple species were colonized, inflammatory cytokine production was synergistically increased or decreased at a certain cell ratio. This was considered to be the cause of the different inflammatory ability and the different responsiveness of the therapeutic drug depending on the case.

Academic Significance and Societal Importance of the Research Achievements

尋常性ざ瘡はグラム陽性細菌であるアクネ菌を起因菌として発症する慢性炎症性の皮膚炎であるが、既存の抗菌薬に不応答な難治性尋常性ざ瘡が存在する。この原因が、アクネ菌のみではなく、多様性型と真菌型であることを明らかにした。また、存在する菌種により炎症誘導能が異なることも判明した。つまり、角栓のマイクロバイオームを解析することで難治性尋常性ざ瘡の個別化治療が可能となる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (24 results)

All 2020 2019 2018 2017

All Journal Article (6 results) (of which Peer Reviewed: 4 results) Presentation (18 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results)

  • [Journal Article] Culture independent approach reveals domination of human-oriented microbes in a pharmaceutical manufacturing facility2019

    • Author(s)
      Kawai Mako、Ichijo Tomoaki、Takahashi Yuji、Noguchi Miyako、Katayama Hirohito、Cho Otomi、Sugita Takashi、Nasu Masao
    • Journal Title

      European Journal of Pharmaceutical Sciences

      Volume: 137 Pages: 104973-104973

    • DOI

      10.1016/j.ejps.2019.104973

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid detection of Lactobacillus crispatus and Lactobacillus iners in vaginal specimens by loop-mediated isothermal amplification2019

    • Author(s)
      Higashide S, Cho O, Matsuda Y, Ogishima D, Kurakado S, Sugita T.
    • Journal Title

      J Microbiol Methods

      Volume: 158 Pages: 18-20

    • DOI

      10.1016/j.mimet.2019.01.011

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Inhibitory effects of Euphorbia supina on Propionibacterium acnes-induced skin inflammation in vitro and in vivo2018

    • Author(s)
      Lim HJ, Jeon YD, Kang SH, Shin MK, Lee KM, Jung SE, Cha JY, Lee HY, Kim BR, Hwang SW, Lee JH, Sugita T, Cho O, Myung H, Jin JS, Lee YM
    • Journal Title

      BMC Complement Altern. Med

      Volume: 18 Issue: 1 Pages: 263-263

    • DOI

      10.1186/s12906-018-2320-8

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] 真菌(病原性酵母)2018

    • Author(s)
      杉田 隆、張 音実、高島昌子
    • Journal Title

      臨床と微生物

      Volume: 45 Pages: 235-240

    • Related Report
      2018 Research-status Report
  • [Journal Article] 病原真菌のゲノム解析から得られる情報2018

    • Author(s)
      杉田 隆、張 音実、倉門早苗
    • Journal Title

      化学療法の領域

      Volume: 45 Pages: 235-240

    • Related Report
      2018 Research-status Report
  • [Journal Article] Inhibition of Propionibacterium acnes lipase activity by the antifungal agent ketoconazole2017

    • Author(s)
      Unno M, Cho O, Sugita T
    • Journal Title

      Microbiol. Immunol

      Volume: 61 Issue: 1 Pages: 42-44

    • DOI

      10.1111/1348-0421.12464

    • NAID

      40021110140

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] チロシンキナーゼ阻害剤ゲフィチニブのアクネ菌を介した作用:ゲフィチニブはアクネ菌による細胞傷害を増強させる2020

    • Author(s)
      飛田 彩香1、張 音実1、杉田 隆
    • Organizer
      日本薬学会第140年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] マイクロバイオームとしてのビフィズス菌とアクネ菌の相互作用2020

    • Author(s)
      渡邉 香那子1、張 音実1、大久保 友隆1、加藤 隼平1、杉田 隆
    • Organizer
      日本薬学会第140年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Candida parapsilosis に対するカルシニューリン阻害薬タクロリムスのアポトーシス作用2019

    • Author(s)
      張音実、髙田慎太郎、杉田 隆
    • Organizer
      第92回日本細菌学会総会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Propidium monoazide処理後の皮膚マイクロバイオームの網羅的解析2019

    • Author(s)
      大久保友隆、張 音実、杉田 隆
    • Organizer
      第92回日本細菌学会総会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 皮膚マイクロバイオームである黄色ブドウ球菌が誘導するIL-18の制御2019

    • Author(s)
      加藤隼平、張 音実、大久保友隆、杉田 隆
    • Organizer
      第63回日本薬学会関東支部大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] PMAを用いた皮膚マイクロバイオーム環境の解明2019

    • Author(s)
      大久保友隆、張 音実、加藤隼平、小笠原祐樹、杉田 隆
    • Organizer
      第63回日本薬学会関東支部大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 正常ヒト表皮角化細胞における真菌Malasseziaと細菌Staphylococcus aureus の相互作用2019

    • Author(s)
      加藤隼平、大久保友隆、張 音実、杉田 隆
    • Organizer
      第63回日本医真菌学会総会・学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 癜風の発症に関与する皮膚常在真菌Malasseiza furfurの二形性変換因子の解析2019

    • Author(s)
      張 音実、須藤綾子、杉田 隆
    • Organizer
      第63回日本医真菌学会総会・学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Localization of Malassezia detected within keratotic plugs and inhibition of Malassezia lipase by the antifungal agent, ketoconazole2019

    • Author(s)
      Cho O, Unno M, Sugita T
    • Organizer
      6th meeting of Asia Pacific Society for Medical Mycology (APSMM)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Comprehensive analysis of the skin microbiome and interaction of Malassezia and bacteria in keratinocytes2019

    • Author(s)
      Sugita T, Cho O
    • Organizer
      6th meeting of Asia Pacific Society for Medical Mycology (APSMM)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Comprehensive Analysis of the Microbiome of the Horny Plug, and Interaction of Malassezia Species and Propionibacterium acnes in Keratinocytes2018

    • Author(s)
      Cho O, Unno M, Sugita T
    • Organizer
      20th Congress of the International Society for Human and Animal Mycology (ISHAM) 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 角栓中の主要菌種MalasseziaとPropionibacterium acnesの正常ヒト表皮角化細胞での相互作用2018

    • Author(s)
      張 音実、海野瑞季、杉田 隆
    • Organizer
      第62回日本医真菌学会総会・学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] 角栓中のマイクロバイオーム解析 -真菌と細菌の正常ヒト表皮角化細胞での相互作用2018

    • Author(s)
      張 音実、海野瑞季、杉田 隆
    • Organizer
      第39回関東医真菌懇話会
    • Related Report
      2018 Research-status Report
  • [Presentation] 角栓中のマイクロバイオーム解析とケラチノサイトへ与える影響: ―Malassezia と Propionibacterium acnes―2018

    • Author(s)
      張 音実、海野 瑞希、杉田 隆
    • Organizer
      日本薬学会第138年会
    • Related Report
      2017 Research-status Report
  • [Presentation] 皮膚上のアシネトバクターは黄色ブドウ球菌の細胞傷害性を増悪させるか2018

    • Author(s)
      井上 瑞菜、張 音実、杉田 隆
    • Organizer
      日本薬学会第138年会
    • Related Report
      2017 Research-status Report
  • [Presentation] ヒト皮膚の真菌マイクロバイオーム -真菌と細菌の相互作用2017

    • Author(s)
      張 音実、井上 瑞菜、海野 瑞季、杉田 隆
    • Organizer
      第61回日本医真菌学会総会・学術集会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 角栓中に存在するMalasseziaとPropionibacerium acnesの直接鏡検と相互作用2017

    • Author(s)
      海野瑞季、張 音実、杉田 隆
    • Organizer
      第61回日本医真菌学会総会・学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] Microbiota in the skin of healthy Japanese individuals between the neonate and adolescent periods2017

    • Author(s)
      Cho O, Matsuda Y, Ogishima D, Inoue M, Sugita T
    • Organizer
      15th International Congress of Mycology and Eukaryotic Microbiology, IUMS 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research

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Published: 2017-04-28   Modified: 2021-02-19  

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