the molecular mechanisms controlling the migration and differentiation of Muse cells
| Project/Area Number |
17K15540
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | Muse細胞 / 多能性幹細胞 / 遊走 / microRNA / 再生医療 |
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| Outline of Final Research Achievements |
To define the molecular mechanisms controlling the migration and differentiation of Muse cells, we evaluated migration ability of Muse cells using serum of mice with CCl4-induced acute hepatic injury. Muse cells exhibited higher migration ability compared with non-Muse cells. Furthermore, MicroRNA microarray analysis revealed that microRNAs changed expression in Muse cells following exposure on hepatitis serum. Effect of differential expressed microRNAs in Muse cells analyzed by introducing these microRNAs into Muse cells. These results established the basics of molecular mechanisms controlling the migration and differentiation of Muse cells.
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| Academic Significance and Societal Importance of the Research Achievements |
生体組織に存在する腫瘍性を持たない多能性幹細胞であるMuse細胞は直接血管内や局所に移植するだけで、傷害組織へ遊走・集積し、組織に応じた細胞に自発的に分化して組織修復を行う。そのため、安全性と簡便性を併せ持つMuse細胞は細胞移植治療に適した細胞であると考えられている。本研究を通じてMuse細胞の組織傷害下において変動するmicroRNAが見出され、未だ明らかでないMuse細胞の遊走・分化制御機構に関わる因子の同定につながる研究の基礎を築けたと考える。
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Quantitative Analysis of SSEA3+ Cells from Human Umbilical Cord after Magnetic Sorting2019
Author(s)
Zikuan Leng, Dongming Sun, Zihao Huang, Iman Tadmori, Ning Chiang, Nikhit Kethidi, Ahmed Sabra, Yoshihiro Kushida, Yu-Show Fu, Mari Dezawa, Xijing He, and Wise Young
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Journal Title
Cell Transplantation
Volume: -
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] S1P-S1PR2 Axis Mediates Homing of Muse Cells into Damaged Heart for Long Lasting Tissue Repair and Functional Recovery After Acute Myocardial Infarction2018
Author(s)
Yamada, Y. Wakao, S. Kushida, Y. Minatoguchi, S. Mikami, A. Higashi, K. Baba, S. Shigemoto, T. Kuroda, Y. Kanamori, H. Amin, M. Kawasaki, M. Nishigaki, K. Taoka, M. Isobe, T. Muramatsu, C. Dezawa, M. Minatoguchi, S.
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Journal Title
Circulation research
Volume: 122
Issue: 8
Pages: 1069-1083
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Beneficial Effects of Systemically Administered Human Muse Cells in Adriamycin Nephropathy.2017
Author(s)
Uchida N, Kushida Y, Kitada M, Wakao S, Kumagai N, Kuroda Y, Kondo Y, Hirohara Y, Kure S, Chazenbalk G, Dezawa M
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Journal Title
J Am Soc Nephrol
Volume: 28
Issue: 10
Pages: 2946-2960
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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