The role of ER-resident transmembrane protein OASIS in cellular senescence
Project/Area Number |
17K15599
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
General medical chemistry
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Research Institution | Hiroshima University |
Principal Investigator |
Asada Rie 広島大学, 医系科学研究科(医), 助教 (70751882)
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Project Period (FY) |
2017-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | 細胞老化 / 小胞体 / DNA損傷 / 核膜 |
Outline of Final Research Achievements |
In this study, we showed a role of ER-resident transcription factor OASIS in cellular senescence. The expression and activation of OASIS are induced by DNA damage in murine primary astrocyte. Investigation of OASIS KO astrocyte revealed that cell cycle arrest and the transition to cellular senescence after DNA damage are repressed compared to WT. Additionally, the expression of a series of senescence-associated secretory phenotype (SASP) related genes is decreased in KO. Collectively, DNA damage induces OASIS expression and activation, and OASIS promotes cellular senescence thorough transcription of cell cycle and SASP related genes.
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Academic Significance and Societal Importance of the Research Achievements |
細胞老化とは、DNA損傷などによってゲノム不安定性を持った細胞が増殖するのを防ぐための癌抑制機構である。本研究課題により、OASISが細胞老化を促進する役割がある事が明らかとなった。過去の解析から、複数種の癌細胞においてOASISの発現が低下していることが明らかとなっている。今後、細胞老化におけるOASISの機能をさらに詳細に解析するともに、癌化との関連性を明らかにしていく事で、新たな視点からの癌治療法開発へと繋がる可能性が期待される。
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Report
(3 results)
Research Products
(12 results)
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[Journal Article] NFAT5 up-regulates expression of the kidney-specific ubiquitin ligase gene Rnf183 under hypertonic conditions in inner-medullary collecting duct cells.2019
Author(s)
Maeoka Y, Wu Y, Okamoto T, Kanemoto S, Guo XP, Saito A, Asada R, Matsuhisa K, Masaki T, Imaizumi K, Kaneko M.
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Journal Title
J Biol Chem.
Volume: 294
Issue: 1
Pages: 101-115
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Shutdown of ER-associated degradation pathway rescues functions of mutant iduronate 2-sulfatase linked to mucopolysaccharidosis type II.2018
Author(s)
Osaki Y, Saito A, Kanemoto S, Kaneko M, Matsuhisa K, Asada R, Masaki T, Orii K, Fukao T, Tomatsu S, Imaizumi K
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Journal Title
Cell Death Dis.
Volume: 9
Issue: 8
Pages: 808-808
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Sec16A, a key protein in COPII vesicle formation, regulates the stability and localization of the novel ubiquitin ligase RNF183.2018
Author(s)
Wu Y, Guo XP, Kanemoto S, Maeoka Y, Saito A, Asada R, Matsuhisa K, Ohtake Y, Imaizumi K, Kaneko M.
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Journal Title
PLoS One
Volume: 13
Issue: 1
Pages: e0190407-e0190407
DOI
Related Report
Peer Reviewed / Open Access
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