Function of NANOG as an inhibition for free c-MYC-dependent apoptosis
| Project/Area Number |
17K15604
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Saitama Medical University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | ES細胞 / c-Myc / アポトーシス |
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| Outline of Final Research Achievements |
In 2011, our lab reported that extinction of Max gene expression in ES cells provoke cell death. Further studies revealed that the MAX-unbound c-MYC in Max-deficient ES cells is responsible factor for the apoptosis exhibited by these cells, and that NANOG functions as a suppressor of apoptosis induction by binding to free c-MYC. NANOG functions to activate the NuRD complex, and MBD3, a component of NuRD, has been reported to bind to c-MYC. Therefore, we examined whether forced expression of MBD3 suppressed the apoptosis exhibited by Max-deficient ES cells and found that apoptosis was suppressed by forced expression of MBD3. In addition, DNA microarray analysis was performed to determine what kind of gene groups are expressed by the forced expression of MBD3.
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| Academic Significance and Societal Importance of the Research Achievements |
MYCタンパク質は、腫瘍化の要因として考えられている一方、古くからMycの強制発現は、アポトーシスを引き起こすと言う、相反する現象にも関与している事が知られていた。本研究はES細胞における、MYCタンパク質によるアポトーシス誘導という、今までにあまり着目されてこなかった研究項目を中心課題に据えた研究ではあったが、c-MYCに関して全く新しい分子指標を見出すことに繋がったと考えている。この得られた知見はES・iPS細胞の特性の根幹をなす『分化多能性・自己増殖性』の理解につながり、しいてはES・iPS細胞を用いた再生医療の安全性の向上につながる事を期待する。
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] Identification of the coiled-coil domain as an essential Mbd3 element for preserving lineage commitment potential of embryonic stem cells2018
Author(s)
Masataka Hirasaki, Atsushi Ueda, Masamitsu N. Asaka, Kousuke Uranishi, Ayumu Suzuki, Masakazu Kohda, Yosuke Mizuno, Yasushi Okazaki, Masazumi Nishimoto, Jafar Sharif, Haruhiko Koseki, Akihiko Okuda
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Journal Title
Stem Cells
Volume: 印刷中
Issue: 9
Pages: 1355-1367
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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