| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
In this study, we established an invasion model of lung cancer and fibroblast cell line using three-dimensional culture. Additionally, we prepared formalin-fixed paraffin-embedded specimens of the invasion model and immunohistochemically confirmed that cancer-associated fibroblast (CAF) -related factors such as α-SMA and CD10 were upregulated in fibroblasts. We also examined the changes of microRNA expression in fibroblasts by in situ hybridization.
In the investigation of surgical specimens, high expression of CAF-related factors such as calumenin and miR-21 in CAFs were a poor prognostic factor. In addition, functional analysis using cell lines also suggested that they interact and promote invasion of lung adenocarcinoma.
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