| Project/Area Number |
17K15642
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Gifu University |
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Principal Investigator |
Saigo Chiemi 岐阜大学, 大学院医学系研究科, 助教 (10547681)
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| Project Period (FY) |
2017-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 滑膜肉腫 / 軟部腫瘍 / 口腔内癌 / Proximity Ligation Assay / PLA / WWOX / TMEM207 / キメラ分子 / proximal ligation / キメラ遺伝子 |
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| Outline of Final Research Achievements |
We performed application of in situ proximal ligation assay to detect the crucial binding of two molecules in malignant tumors. First, we tried to detect the SS18-SSX fusion protein in synovial sarcoma. Subsequently, we detected the binding of tumor suppressor factor WWOX and a small transmembrane protein, TMEM207, in oral squamous cell carcinoma.
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| Academic Significance and Societal Importance of the Research Achievements |
滑膜肉腫で、SS18-SSX融合遺伝子産物の検出、可視化に成功した。これは染色体転座を有する腫瘍(悪性リンパ腫を含む)で通常ホルマリン固定病理切片で、融合遺伝子産物の特異的検出が可能になる道筋をつけたと考える。また、口腔内癌でWWOXとTMEM207の結合を検出しえたことで、悪性腫瘍細胞での腫瘍抑制因子機能を不活化する異常たんぱく質発現が病理組織切片で検出可能なことを示したと考える。
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