An application of myosin 1B, a newly identified human pericytic marker, to diagnostic pathology

• Project/Area Number: 17K15643
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Human pathology
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: Meguro Shiori 浜松医科大学, 医学部, 助教 (40724290)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2019: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
• Keywords: pericyte / 血管平滑筋細胞 / perivascular tumor / myosin 1B / Pericyte / 病理学

Outline of Final Research Achievements

In our previous study, we identified myosin 1B (MYO1B) as a new pericyte marker, and we have reported that vascular mural cells were classified into three types of cells; α-SMA(+)/MYO1B(+)/hCD(-) pericytes, α-SMA(+)/MYO1B(-)/hCD(+) vascular smooth muscle cells, and α-SMA(+)/MYO1B(+)/hCD(+) vascular mural cells with intermediate features.
The aim of this study was the application of MYO1B to diagnostic pathology. We found a soft tissue tumor entirely composed of α-SMA(+)/MYO1B(+)/hCD(-) tumor cells, indicating that the tumor was a perivascular myoid tumor with definitive pericytic differentiation.
Furthermore, we reported a case of a nasal glomus tumor with diffuse and strong synaptophysin expression.

Academic Significance and Societal Importance of the Research Achievements

MYO1BとhCDを組み合わせることで、ヒトホルマリン固定パラフィン包埋材料において、免疫組織化学的にpericyteへの分化を検討できるようになった。MYO1Bを診断病理学に応用し、皮下腫瘍および軟部腫瘍のpericyteへの分化を検討したところ、pericyteのみから構成されると考えられる腫瘍を見出し、報告することができた。
その他にも、鼻領域のsinonasal glomangiopericytomaなどpericyteへの分化を検討したい腫瘍において、MYO1Bは有用であると考えられる。

Research Products

• [Journal Article] Nasal glomus tumor: A rare nasal tumor with diffuse and strongly positive synaptophysin expression (2019)
  • Author(s): Meguro Shiori、Kusama Yukiko、Matsushima Sayomi、Yagi Haruna、Kawasaki Hideya、Kosugi Isao、Tsuchida Takashi、Baba Satoshi、Enomoto Yasunori、Hosokawa Seiji、Iwashita Toshihide
  • Journal Title: Pathology International Volume: 69 Issue: 11 Pages: 672-674
  • DOI: 10.1111/pin.12866
  • Peer Reviewed / Open Access
• [Journal Article] Immunohistochemical examination using the pericyte marker myosin 1B in a perivascular myoid tumor of soft tissue with definitive pericytic differentiation. (2018)
  • Author(s): Meguro S, Matsushima S, Enomoto Y, Kawasaki H, Kosugi I, Tsuchida T, Baba S, Fukamizu H, Yamato Y, Iwashita T.
  • Journal Title: Patholology International Volume: 69 Issue: 4 Pages: 246-248
  • DOI: 10.1111/pin.12777
  • Peer Reviewed / Open Access