Molecular analysis of intracranial germ cell tumors based on histopathological background
| Project/Area Number |
17K15659
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | National Cancer Center Japan |
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Principal Investigator |
satomi kaishi 国立研究開発法人国立がん研究センター, 中央病院, 医員 (10633977)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 胚細胞腫 / 中枢神経系 / DNAメチル化 / コピー数異常 / FISH / 予後 / 脳腫瘍 / コピー数 / 頭蓋内胚細胞腫 / DNAメチル化アレイ / 胚腫 / 免疫組織化学 / 中枢神経系胚細胞腫 / 組織型 / 分子生物学的解析 / レーザーマイクロダイセクション |
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| Outline of Final Research Achievements |
To elucidate the pathogenesis of intracranial germ cell tumors (iGCTs). We are analyzed a copy number analysis by multi-institutional study.
Firstly, we analyzed DNA methylation profile in 83 iGCTs and 6 normal control samples using Infinium Human Methylation 450K BeadChip array and found 12p gain in 37.3% of iGCTs. Then, 58 iGCTs with clinicopathological information were analyzed for progression-free survival (PFS) and overall survival (OS). Both PFS and OS were significantly worse in iGCTs with 12p gain. Lastly, fluorescence in situ hybridization (FISH) study was carried out on 3 mixed iGCT cases. Different histological components in each mixed GCT shared the same 12p copy number status within each mixed GCT case.
Gain of 12p can be a molecular marker to predict prognosis and an early event in tumorigenesis prior to histological differentiation in iGCTs.
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| Academic Significance and Societal Importance of the Research Achievements |
頭蓋内胚細胞腫(iGCT)は、悪性度の異なる6つの組織型から構成され、複数の組織型が同一腫瘍内に混在することがある。本研究では、12番染色体短腕のgain (12p gain)は予後不良な組織型で高頻度にみられることが明らかとなり、iGCT患者の予後予測マーカーとして臨床応用可能と考えられる。
また、12p gainに加え倍数性（染色体セットの数）の異常は、同一腫瘍内であれば組織型が異なっても共通することが明らかとなり、これらの異常がiGCTの腫瘍発生の比較的早期に発生するイベントであることが示唆され、病態解明の一助となる。
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] Intratumoural immune cell landscape in germinoma reveals multipotent lineages and exhibits prognostic significance2019
Author(s)
Takami H, Fukushima S, Aoki K, Satomi K, Narumi K, Hama N, Matsushita Y, Fukuoka K, Yamasaki K, Nakamura T, Mukasa A, Saito N, Suzuki T, Yanagisawa T, Nakamura H, Sugiyama K, Tamura K, Maehara T, Nakada M, Nonaka M, Asai A, et al.
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Journal Title
Neuropathology and Applied Neurobiology
Volume: -
Issue: 2
Pages: 111-124
DOI
Related Report
Peer Reviewed / Open Access
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