Screening of in vivo essential factor inhibiters for multi-drug resistant bacteria
Project/Area Number |
17K15688
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Bacteriology (including mycology)
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Research Institution | Sapporo Medical University |
Principal Investigator |
Sato Toyotaka 札幌医科大学, 医学部, 助教 (30756474)
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Project Period (FY) |
2017-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 細菌 / 薬剤耐性菌 / 生体内菌発育必須因子 / vivoEF / 多剤耐性 / 感染部位特異的治療 / 敗血症 / 血流感染症 / 多剤耐性菌 / 抗菌薬 / 化合物スクリーニング / 感染症 |
Outline of Final Research Achievements |
In this study, we focused on bacterial factors that are essential for their bacterial growth and survival (vivoEF) in vivo(infected sites such as tissues and blood), but not for in vitro, from a view of emergence and spread of multidrug-resistant bacteria that is considered to be a problem in the future. We screened and identified vivoEF inhibitors. We also identified their targets in bacteria, and evaluated vivoEF effects using infectious models in mouse. As a result, a plurality of compounds showing antibacterial activity only in blood were identified, and it was clarified that the compounds suppress the production of bacterial capsule. From the above, we obtained scientific findings on new treatments for bacterial bloodstream infections such as sepsis in this study.
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Academic Significance and Societal Importance of the Research Achievements |
敗血症といった細菌性血流感染症による死亡率は増加の一途をたどっており、多剤耐性菌はその治療を一層困難にしている。よって本研究で明らかにした、『生体内菌発育必須因子(vivoEF) 』とその阻害剤の同定から得た科学的知見は、多剤耐性化や新たな耐性菌を生み出さない新たな感染症治療戦略の確立に寄与する重要な意義を持つと考えられる。
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Report
(3 results)
Research Products
(26 results)
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[Journal Article] Complete Genome Sequence of Multidrug-Resistant Streptococcus pneumoniae Serotype 19F Isolated from an Invasive Infection in Sapporo, Japan2017
Author(s)
Sato T, Ohkoshi Y, Wada T, Fukushima Y, Murabayashi H, Takakuwa Y, Nishiyama K, Shiraishi T, Nakajima C, Suzuki Y, Yokota SI
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Journal Title
Genome Announc
Volume: 5
Issue: 44
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Novel antimicrobial activities of a peptide derived from a Japanese soybean fermented food, Natto, against Streptococcus pneumoniae and Bacillus subtilis group strains.2017
Author(s)
Manabu Kitagawa, Tsukasa Shiraishi, Soh Yamamoto, Ryosuke Kutomi, Yasuo Ohkoshi, Toyotaka Sato, Hideki Wakui, Hideaki Itoh, Atsushi Miyamoto, Shin-ichi Yokota.
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Journal Title
AMB Express
Volume: 7
Issue: 1
Pages: 127-127
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Interleukin-27 Enhances the Potential of Reactive Oxygen Species Generation from Monocyte-derived Macrophages and Dendritic cells by Induction of p47phox.2017
Author(s)
Bharatwaj Sowrirajan, Yoshiro Saito, Deepak Poudyal, Qian Chen, Hongyan Sui, Suk See DeRavin, Hiromi Imamichi, Toyotaka Sato, Douglas B. Kuhns, Noriko Noguchi, Harry L. Malech, H. Clifford Lane and Tomozumi Imamichi
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Journal Title
Scientific Reports
Volume: 7
Issue: 1
Pages: 43441-43441
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] In vivo generation of extensively drug-resistant Klebsiella pneumoniae via a disrupting mutation in the DNA repair enzyme MutS2018
Author(s)
T. Sato, M. Shinagawa, S. Nishijima, Y. Fukushima, C. Nakajima, H. Honda, T. Shiraishi, K. Kuronuma, H. Takahashi, Y. Suzuki, S. Takahashi, Y. SI. Yokota
Organizer
ASM Microbe 2018
Related Report
Int'l Joint Research
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[Presentation] Genetic analysis of high-level β-lactamase-negative ampicillin-resistant strains and in vitro-selected fluoroquinolone-resistant mutants of Haemophilus influenzae2018
Author(s)
Honda H, Sato T, Shinagawa M, Fukushima Y, Nakajima C, Suzuki Y, Shiraishi T, Kuronuma K, Takahashi S, Takahashi H, Yokota SI
Organizer
ASM Microbe 2018
Related Report
Int'l Joint Research
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[Presentation] 大腸菌臨床分離株におけるepidemic clone, ST131のコリスチン及びチゲサイクリン耐性2018
Author(s)
Toyotaka Sato, Masaru Usui, Masaaki Shinagawa, Akira Fukuda, Hiroyuki Honda, Tsukasa Shiraishi, Hiroki Takahashi, Yutaka Tamura, Satoshi Takahashi, Shin-ichi Yokota
Organizer
第91回日本細菌学会総会
Related Report
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[Presentation] Tigecycline non-susceptibility occurs exclusively in fluoroquinolone-resistant Escherichia coli clinical isolates, including the major multidrug-resistant lineages O25b:H4-ST131-H30R and O1-ST6482017
Author(s)
T. Sato, Y. Suzuki, T. Shiraishi, H. Honda, M. Shinagawa, S. Yamamoto, N. Ogasawara, S. Takahashi, Y. Tamura, SI. Yokota
Organizer
ASM Microbe 2017
Related Report
Int'l Joint Research
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