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Evaluation of cell-to-cell transmission in Hepatitis B Virus

Research Project

Project/Area Number 17K15708
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Virology
Research InstitutionMeiji University (2019-2020)
National Institute of Infectious Diseases (2017-2018)

Principal Investigator

TAKEUCHI Junko S.  明治大学, 研究・知財戦略機構(生田), 特任講師 (80647488)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
KeywordsB型肝炎ウイルス / Cell-to-cell感染 / ウィルス / HBV
Outline of Final Research Achievements

Viruses such as human immunodeficiency virus (HIV) can spread via two types of replication strategies: cell-free infection and cell-to-cell infection. Viral cell-to-cell infection, in which viral particles transmit to adjacent uninfected cells, has shown to be more efficient than infection by cell-free virus particles. However, it is still controversial whether Hepatitis B virus (HBV) can propagate via cell-to-cell infection. To address this question, HBV producer cells were co-cultured with HBV susceptible cells in the presence or absence of
neutralizing antibodies (nAbs). In the absence of nAbs, HBV propagates by both cell-free and cell-to-cell infections. While, in the presence of nAbs, cell-free transmission is inhibited, thereby allowing HBV to replicate only by the cell-to-cell infection. Our co-culture experiments demonstrated that HBV may spread by the cell-to-cell infection mode.

Academic Significance and Societal Importance of the Research Achievements

HIV-1の研究においては、cell-free感染では低コピー数のウイルスが細胞に感染するのに対し、cell-to-cell感染では大量のウイルスが細胞に受け渡されるため、薬剤感受性が低いと報告されている。
HBVにおいても、現行の治療法では持続感染者からウイルスを完全に排除できないが、HBV cell-to-cell 感染と薬剤感受性についての関連は不明である。本研究は、「抗HBV剤がcell-free 感染だけでなくcell-to-cell 感染も標的としているか?」を評価できるスクリーニング系開発の一助となることが期待される。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (18 results)

All 2021 2020 2019 2018 2017 Other

All Journal Article (8 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 8 results,  Open Access: 5 results) Presentation (6 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results) Remarks (4 results)

  • [Journal Article] MafF is an antiviral host factor that suppresses transcription from Hepatitis B Virus core promoter2021

    • Author(s)
      Ibrahim Marwa K.、Abdelhafez Tawfeek H.、Takeuchi Junko S.、Wakae Kosho、Sugiyama Masaya、Tsuge Masataka、Ito Masahiko、Watashi Koichi、El Kassas Mohamed、Kato Takanobu、Murayama Asako、Suzuki Tetsuro、Chayama Kazuaki、Shimotohno Kunitada、Muramatsu Masamichi、Aly Hussein H.、Wakita Takaji
    • Journal Title

      Journal of Virology

      Volume: 「-」 Issue: 15

    • DOI

      10.1128/jvi.00767-21

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Dual Agonist of Farnesoid X Receptor and G Protein‐coupled Receptor TGR5 Inhibits Hepatitis B Virus Infection in Vitro and in Vivo2021

    • Author(s)
      Ito Kiyoaki、Okumura Akinori、Takeuchi Junko S. 、Watashi Koichi、Inoue Rieko、Yamauchi Taeko、Sakamoto Kazumasa、Yamashita Yukiko、Iguchi Yusuke、Une Mizuho、Wakita Takaji、Umezawa Kazuo、Yoneda Masashi
    • Journal Title

      Hepatology

      Volume: 74(1) Issue: 1 Pages: 83-98

    • DOI

      10.1002/hep.31712

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Single Adaptive Mutation in Sodium Taurocholate Cotransporting Polypeptide Induced by Hepadnaviruses Determines Virus Species Specificity2018

    • Author(s)
      Takeuchi Junko S.、Fukano Kento、Iwamoto Masashi、Tsukuda Senko、Suzuki Ryosuke、Aizaki Hideki、Muramatsu Masamichi、Wakita Takaji、Sureau Camille、Watashi Koichi
    • Journal Title

      Journal of Virology

      Volume: 93 Issue: 5 Pages: 04132-18

    • DOI

      10.1128/jvi.01432-18

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Detection of pathogenic bacteria in the blood from sepsis patients using 16S rRNA gene amplicon sequencing analysis.2018

    • Author(s)
      Watanabe N, Kryukov K, Nakagawa S, Takeuchi JS, Takeshita M, Kirimura Y, Mitsuhashi S, Ishihara T, Aoki H, Inokuchi S, Imanishi T, Inoue S
    • Journal Title

      PLoS One

      Volume: 13 Issue: 8 Pages: e0202049-e0202049

    • DOI

      10.1371/journal.pone.0202049

    • NAID

      120006826803

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Experimental adaptive evolution of simian immunodeficiency virus SIVcpz to pandemic human immunodeficiency virus type 1 using a humanized mouse model2018

    • Author(s)
      Kei Sato*, Naoko Misawa, Junko S Takeuchi, Tomoko Kobayashi, Taisuke Izumi, Hirofumi Aso, Shumpei Nagaoka, Keisuke Yamamoto, Izumi Kimura, Yoriyuki Konno, Yusuke Nakano & Yoshio Koyanagi. (*Correspondence)
    • Journal Title

      Journal of Virology

      Volume: 92 Issue: 4

    • DOI

      10.1128/jvi.01905-17

    • Related Report
      2018 Research-status Report 2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] A portable system for rapid bacterial composition analysis using a nanopore-based sequencer and laptop computer2017

    • Author(s)
      Satomi Mitsuhashi, Kirill Kryukov, So Nakagawa, Junko S. Takeuchi, Yoshiki Shiraishi, Koichiro Asano, Tadashi Imanishi
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 5657-5657

    • DOI

      10.1038/s41598-017-05772-5

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Feline immunodeficiency virus evolutionarily acquires two proteins, Vif and protease, capable of antagonizing feline APOBEC3.2017

    • Author(s)
      Yoshikawa R, Takeuchi J, Yamada E, Nakano Y, Misawa N, Kimura Y, Ren F, Miyazawa T, Koyanagi Y, Sato K.
    • Journal Title

      J Virol

      Volume: JVI.00250-17 Issue: 11

    • DOI

      10.1128/jvi.00250-17

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cyclosporin derivatives inhibit hepatitis B virus entry without interfering with NTCP transporter activity.2017

    • Author(s)
      Shimura S, Watashi K, Fukano K, Peel M, Sluder A, Kawai F, Iwamoto M, Tsukuda S, Takeuchi S J, Miyake T, Sugiyama M, Ogasawara Y, Park SY, Tanaka Y, Kusuhara H, Mizokami M, Sureau C, Wakita T
    • Journal Title

      J Hepatol

      Volume: 66 Issue: 4 Pages: 685-692

    • DOI

      10.1016/j.jhep.2016.11.009

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Adaptive evolution in hepatitis B virus (HBV) receptor2020

    • Author(s)
      Junko S TAKEUCHI
    • Organizer
      The 43rd Annual Meeting of the Molecular Biology Society of Japan, To be held Online
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] A Single Adaptive Mutation in Sodium Taurocholate Cotransporting Polypeptide (NTCP) Induced by Hepadnaviruses Determines Virus Species Specificity.2019

    • Author(s)
      Junko S TAKEUCHI
    • Organizer
      7th Japan-Taiwan-Korea HBV Research Symposium, Tokyo, JAPAN
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヘパドナウイルスは宿主進化の選択圧となったか2019

    • Author(s)
      竹内(柴田)潤子
    • Organizer
      第21回 日本進化学会(札幌)
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] HBV-related hepadnaviruses drive an adaptive evolution in a host receptor2019

    • Author(s)
      Junko S TAKEUCHI
    • Organizer
      The 67th Annual Meeting of the Japanese Society for Virology, Tokyo, JAPAN
    • Related Report
      2019 Research-status Report
  • [Presentation] 教育講演:ウイルスの進化とその解析法に関して2019

    • Author(s)
      竹内(柴田)潤子.
    • Organizer
      第7回肝炎ウイルス研修会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] A Single Adaptive Mutation in Sodium Taurocholate Cotransporting Polypeptide (NTCP) Induced by Hepadnaviruses Determines Virus Species Specificity2019

    • Author(s)
      Junko S TAKEUCHI
    • Organizer
      7th Japan-Taiwan-Korea HBV Research Symposium
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Remarks] 国立感染症研究所ウイルス第二部

    • URL

      https://www.niid.go.jp/niid/ja/from-vir2.html

    • Related Report
      2018 Research-status Report
  • [Remarks] 渡士研

    • URL

      https://www.watashi-lab.net/

    • Related Report
      2018 Research-status Report
  • [Remarks]

    • URL

      https://www.niid.go.jp/niid/ja/basic-science/virology/8506-virology-2018-6.html

    • Related Report
      2018 Research-status Report
  • [Remarks] 国立感染症研究所 ウイルス第二部

    • URL

      https://www.niid.go.jp/niid/ja/from-vir2.html

    • Related Report
      2017 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2024-12-25  

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