Evaluation of cell-to-cell transmission in Hepatitis B Virus
| Project/Area Number |
17K15708
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Virology
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| Research Institution | Meiji University (2019-2020)
National Institute of Infectious Diseases (2017-2018) |
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Principal Investigator |
TAKEUCHI Junko S. 明治大学, 研究・知財戦略機構(生田), 特任講師 (80647488)
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | B型肝炎ウイルス / Cell-to-cell感染 / ウィルス / HBV |
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| Outline of Final Research Achievements |
Viruses such as human immunodeficiency virus (HIV) can spread via two types of replication strategies: cell-free infection and cell-to-cell infection. Viral cell-to-cell infection, in which viral particles transmit to adjacent uninfected cells, has shown to be more efficient than infection by cell-free virus particles. However, it is still controversial whether Hepatitis B virus (HBV) can propagate via cell-to-cell infection. To address this question, HBV producer cells were co-cultured with HBV susceptible cells in the presence or absence of
neutralizing antibodies (nAbs). In the absence of nAbs, HBV propagates by both cell-free and cell-to-cell infections. While, in the presence of nAbs, cell-free transmission is inhibited, thereby allowing HBV to replicate only by the cell-to-cell infection. Our co-culture experiments demonstrated that HBV may spread by the cell-to-cell infection mode.
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| Academic Significance and Societal Importance of the Research Achievements |
HIV-1の研究においては、cell-free感染では低コピー数のウイルスが細胞に感染するのに対し、cell-to-cell感染では大量のウイルスが細胞に受け渡されるため、薬剤感受性が低いと報告されている。
HBVにおいても、現行の治療法では持続感染者からウイルスを完全に排除できないが、HBV cell-to-cell 感染と薬剤感受性についての関連は不明である。本研究は、「抗HBV剤がcell-free 感染だけでなくcell-to-cell 感染も標的としているか？」を評価できるスクリーニング系開発の一助となることが期待される。
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Detection of pathogenic bacteria in the blood from sepsis patients using 16S rRNA gene amplicon sequencing analysis.2018
Author(s)
Watanabe N, Kryukov K, Nakagawa S, Takeuchi JS, Takeshita M, Kirimura Y, Mitsuhashi S, Ishihara T, Aoki H, Inokuchi S, Imanishi T, Inoue S
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Journal Title
PLoS One
Volume: 13
Issue: 8
Pages: e0202049-e0202049
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Experimental adaptive evolution of simian immunodeficiency virus SIVcpz to pandemic human immunodeficiency virus type 1 using a humanized mouse model2018
Author(s)
Kei Sato*, Naoko Misawa, Junko S Takeuchi, Tomoko Kobayashi, Taisuke Izumi, Hirofumi Aso, Shumpei Nagaoka, Keisuke Yamamoto, Izumi Kimura, Yoriyuki Konno, Yusuke Nakano & Yoshio Koyanagi. (*Correspondence)
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Journal Title
Journal of Virology
Volume: 92
Issue: 4
DOI
Related Report
Peer Reviewed
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[Journal Article] Feline immunodeficiency virus evolutionarily acquires two proteins, Vif and protease, capable of antagonizing feline APOBEC3.2017
Author(s)
Yoshikawa R, Takeuchi J, Yamada E, Nakano Y, Misawa N, Kimura Y, Ren F, Miyazawa T, Koyanagi Y, Sato K.
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Journal Title
J Virol
Volume: JVI.00250-17
Issue: 11
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Cyclosporin derivatives inhibit hepatitis B virus entry without interfering with NTCP transporter activity.2017
Author(s)
Shimura S, Watashi K, Fukano K, Peel M, Sluder A, Kawai F, Iwamoto M, Tsukuda S, Takeuchi S J, Miyake T, Sugiyama M, Ogasawara Y, Park SY, Tanaka Y, Kusuhara H, Mizokami M, Sureau C, Wakita T
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Journal Title
J Hepatol
Volume: 66
Issue: 4
Pages: 685-692
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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