Characterization of HBV infection mediated by extracellular vesicles
| Project/Area Number |
17K15709
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Virology
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| Research Institution | Tokyo Metropolitan Institute of Medical Science |
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Principal Investigator |
SANADA Takahiro 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 研究員 (60721272)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | HBV / エキソソーム / ウイルス / B型肝炎ウイルス |
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| Outline of Final Research Achievements |
We investigated the extracellular vesicle (EV)-mediated transmission of hepatitis B virus (HBV) infection. Purified EVs from sera of HBV-infected chimeric mice with human liver contain viral DNA and to be capable or transmitting HBV DNA to naive hepatocytes. These HBV-DNA transmitting EVs were resistant to antibody neutralization. To establish a protective vaccine which can block HBV infection via EVs, we evaluated the effects of intranasal vaccination with HBs and HBc. Intranasal immunization with HBs-L and HBc formulated with CVP strongly induced cellular and humoral immune responses.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究により、エキソソームがHBVの産生機構および感染機構に関与していることが示唆された。本研究をさらに進めていくことで、現在問題となっているワクチン接種者でのHBV感染のメカニズムの解明につながっていくものと考えられる。また細胞性免疫を効率的に誘導できる免疫法に関して、基礎的なデータを得ることができた。本研究によって得られたデータは、エキソソームを介した感染を防御できる予防法の構築につながるものと考えられる。
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Interferon-β response is impaired by hepatitis B virus infection in Tupaia belangeri2017
Author(s)
Kayesh MEH, Ezzikouri S, Chi H, Sanada T, Yamamoto N, Kitab B, Haraguchi T, Matsuyama R, Nkogue CN, Hatai H, Miyoshi N, Murakami S, Tanaka Y, Takano JI, Shiogama Y, Yasutomi Y, Kohara M, Tsukiyama-Kohara K
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Journal Title
Virus Research
Volume: 237
Pages: 47-57
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Presentation] HBs-large protein vaccination via intranasal route with carboxyl vinyl polymer induces strong neutralizing antibody and anti-HBs IgA2018
Author(s)
SanadaT, Kayesh MEH, Chi H, Yamamoto N, Tsukiyama-Kohara K, Yoshida O, Hiasa Y, Hasegawa H, Sugiyama M, Mizokami M, Oda Y, Goh Y, Miyazaki T, Kamishita T, Kohara M.
Organizer
2018 International HBV Meeting: The Molecular Biology of Hepatitis B Viruses
Related Report
Int'l Joint Research
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[Presentation] HBs-large protein vaccination via intranasal route with carboxyl vinyl polymer induces strong neutralizing antibody and anti-HBs IgA2018
Author(s)
SanadaT, Kayesh MEH, Chi H, Yamamoto N, Tsukiyama-Kohara K, Yoshida O, Hiasa Y, Hasegawa H, Sugiyama M, Mizokami M, Oda Y, Goh Y, Miyazaki T, Kamishita T, Kohara M.
Organizer
第66回日本ウイルス学会学術集会
Related Report
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