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Detection of HER family dimers and prediction of anticancer drug efficacy based on single particle imaging

Research Project

Project/Area Number 17K15767
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Laboratory medicine
Research InstitutionTohoku University

Principal Investigator

Kitamura Narufumi  東北大学, 医学系研究科, 講師 (50624912)

Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords乳がん / HERファミリー / ヘテロダイマー / 蛍光エネルギー移動 / ヘテロ二量体 / 蛍光1粒子イメージング / FRET
Outline of Final Research Achievements

We found that the HER2 / HER3 heterodimer can be detected by a technology developed using FRET between fluorescent nanoparticles developed independently. Furthermore, in order to clarify whether this physical phenomenon correctly detects the dimer, the efficiency of energy transfer is studied using a sample in which the distance between fluorescent particles is controlled, and the energy transfer is generally performed only when the dimer is formed. However, some of the particles that are the donor of the fluorescence and the particles that are the acceptor do not correspond on a one-to-one basis, or some of the particles that only exist in the vicinity without forming a dimer were detected. It is necessary to continue to consider the method of evaluation excluding

Academic Significance and Societal Importance of the Research Achievements

本研究は、HER2標的抗体医薬の分子機能阻害効果を予測・診断する技術を開発することを目的とした基礎研究である。今回得られた成果は、乳がんの診断における標的分子の、生体内における分布や形態について様々な知見を得るのに貢献した。今後、診断技術開発における標的分子の解析の研究に重要な知見を与えるものであり、将来的に1分子レベルで標的分子を可視化することが可能となれば、分子標的薬治療の効果予測や創薬支援技術への応用が期待できる。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (9 results)

All 2019 2018 2017

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (8 results) (of which Int'l Joint Research: 5 results)

  • [Journal Article] Quantitative analyses of amount and localization of radiosensitizer gold nanoparticles interacting with cancer cells to optimize radiation therapy2019

    • Author(s)
      Hatoyama Keiichiro、Kitamura Narufumi、Takano-Kasuya Mayumi、Tokunaga Masayuki、Oikawa Takahiro、Ohta Mineto、Hamada Yoh、Tada Hiroshi、Kobayashi Yoshio、Kamei Takashi、Gonda Kohsuke
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 508 Issue: 4 Pages: 1093-1100

    • DOI

      10.1016/j.bbrc.2018.12.016

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Quantitative Analysis of the Mechanism for Radiation Therapy Enhanced by Gold Nanoparticles as Radiosensitizers2018

    • Author(s)
      Narufumi Kitamura, Keiichiro Hatoyama, Mayumi Takano-Kasuya, Masayuki Tokunaga, Yoh Hamada, Takashi Kamei, Kohsuke Gonda
    • Organizer
      12th International Symposium on Nanomedicine
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Radiosensitizer-assisted cancer radiotherapy by using tumor targeting antigen-conjugated gold nanoparticles2018

    • Author(s)
      Keiichiro Hatoyama, ○Narufumi Kitamura, Mayumi Takano-Kasuya, Yoh Hamada, Takashi Kamei, Kohsuke Gonda
    • Organizer
      The 6th Japan-China Symposium on Nanomedicine
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Quantification of protein heterodimers by using fluorescent nanoparticles for breast cancer diagnosis2018

    • Author(s)
      Narufumi Kitamura, Hiroshi Tada, Kohsuke Gonda
    • Organizer
      第77回日本癌学会学術総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] タンパク質二量体の定量のための評価法の開発: 粒子間蛍光エネルギー移動2018

    • Author(s)
      北村成史, 多田寛, 古澤直子, 中野寧, 権田幸祐
    • Organizer
      ナノ学会第16回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Advanced radiotherapy by using tumor-targeting gold nanoparticles2018

    • Author(s)
      Keiichiro Hatoyama, Narufumi Kitamura, Mayumi Takano-Kasuya, Yoh Hamada, Takashi Kamei, Kohsuke Gonda
    • Organizer
      The 98th CSJ Annual Meeting
    • Related Report
      2018 Annual Research Report
  • [Presentation] Development of Quantitative Evaluation Method of Protein Heterodimers2017

    • Author(s)
      Narufumi Kitamura, Hiroshi Tada, Kohsuke Gonda
    • Organizer
      9th A3 Foresight Symposium
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Inter-nanoparticle fluorescence energy transfer for quantitative evaluation of protein heterodimers2017

    • Author(s)
      Narufumi Kitamura, Hiroshi Tada, Naoko Furusawa, Yasushi Nakano, Kohsuke Gonda
    • Organizer
      11th International Symposium on Nanomedisine
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Development of Quantitative Evaluation Method of Protein Heterodimers2017

    • Author(s)
      Narufumi Kitamura, Hiroshi Tada, Kohsuke Gonda
    • Organizer
      5th China-Japan Symposium on Nanomedicine
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research

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Published: 2017-04-28   Modified: 2020-03-30  

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