| Project/Area Number |
17K15769
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Chiba University |
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Principal Investigator |
Mashimo Yoichi 千葉大学, 大学院医学研究院, 技術専門職員 (90422253)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | インフルエンザ菌 / 侵襲性感染症 / 無莢膜株 / 全ゲノムシーケンス / 次世代シーケンサー / 感染症 / ゲノム / 細菌 |
|---|
| Outline of Final Research Achievements |
In this study, we determined the whole genome sequences of nontypeable Haemophilus influenzae (NTHi) strains to identify invasive factor(s) in invasive NTHi infections. Highly accurate whole genome sequences of NTHi strains were obtained using long-read next-generation sequencing platforms (PacBio) and mutations in each strain were detected by comparison with reference sequences published in NCBI as complete genomes. In future studies, we plan to collect more whole genome sequences of H. influenzae strains to identify important factors related to, such as invasiveness, pathogenicity, toxicity and antimicrobial susceptibility.
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| Academic Significance and Societal Importance of the Research Achievements |
Hibワクチンの普及以降、NTHi株による侵襲性感染症の報告が増加しており、病原菌の侵襲性に関与する因子には未解明の部分が多く存在している。また、薬剤耐性菌の増加も問題であり、その対策が重要となっている。対策としてまず、原因となる起炎菌について正確に知るということが挙げられ、正確な全ゲノム配列を決定することが診断法、治療薬、ワクチンの開発などに有用となると期待される。
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