The development of diagnostic tool for severe fever with thrombocytopenia syndrome (SFTS) in a SFTS-endemic area in Japan.
| Project/Area Number |
17K15778
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | SFTS / diagnostic tools / Double-antigen ELISA / 重症熱性血小板減少症候群 / SFTS抗体 / SFTS抗原 / マダニ媒介感染症 / ELISA / イムノクロマトグラフ法 / ムノクロマトグラフ法 / 臨床検査医学 |
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| Outline of Final Research Achievements |
The aim of this study was to develop the useful diagnostic tool for severe fever with thrombocytopenia syndrome (SFTS) in daily clinical practice. We prepared recombinant SFTS virus-associated protein such as nucleoprotein (NP) and nonstructural S segment (NS) protein. Anti- recombinant NP (rNP) antibody was purified from serum of immunized rabbit with rNP. Double-antigen enzyme-linked immunosorbent assay (ELISA) using rNP detected anti-NP antibody in serum obtained from patients with early onset of SFTS. This assay showed that anti-NP antibody increased in serum within 3 or 4 days after SFTS virus infection. Although immunochromatographic assay (ICA) to detect SFTS virus antigen was also developed using anti-rNP rabbit antibody, the performance of ICA was unstable. In future study, it is necessary to improve the sensitivity of ICA for early diagnosis of SFTS. Double-antigen ELISA may be useful diagnostic tool for SFTS virus infection in a SFTS-endemic area in Japan.
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| Academic Significance and Societal Importance of the Research Achievements |
致死率の高い感染症である重症熱性血小板減少症候群（SFTS）には有効な治療法がない．近年，SFTS発症早期にファビピラビルを投薬することにより，SFTS患者の回復が期待できることが示されている．本研究で確立したdouble-antigen ELISA法は，SFTSの早期診断に有用と考えられ，早期治療介入に貢献し死亡率を低減できる可能性がある．
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Report
(4 results)
Research Products
(7 results)
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[Presentation] 重症熱性血小板減少症候群(SFTS)と日本紅斑熱(JSF)における鑑別点の検討.2019
Author(s)
川口剛, 木村賢俊, 川田千紘, 岩尾浩昭, 河野彩子, 仮屋裕美, 松田基弘, 宮内俊一, 梅北邦彦, 高城一郎, 岡山昭彦.
Organizer
第93回日本感染症学会総会・学術講演会.
Related Report
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[Presentation] 重症熱性血小板減少症候群（SFTS）における凝固能異常.2018
Author(s)
川口剛, 川田千紘, 力武雄幹, 力武真央, 岩尾浩昭, 相澤彩子, 仮屋裕美, 松田基弘, 宮内俊一, 梅北邦彦, 高城一郎, 岡山昭彦
Organizer
第92回日本感染症学会講演会 第66回日本化学療法学会総会合同学会
Related Report
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[Presentation] 致死性不整脈を合併した重症熱性血小板減少症候群の一例.2018
Author(s)
岩尾浩昭, 川口剛, 木村賢俊, 川田千紘, 力武真央, 相澤彩子, 仮屋裕美, 松田基弘, 宮内俊一, 梅北邦彦, 高城一郎, 岡山昭彦
Organizer
第1回SFTS研究会・学術集会
Related Report
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[Presentation] 重症熱性血小板減少症候群(SFTS) における神経学的異常.2018
Author(s)
川口剛, 木村賢俊, 川田千紘, 力武真央, 岩尾浩昭, 相澤彩子, 仮屋裕美, 松田基弘, 宮内俊一, 梅北邦彦, 高城一郎, 岡山昭彦
Organizer
第1回SFTS研究会・学術集会
Related Report
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