| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
The aim of this study was to develop the useful diagnostic tool for severe fever with thrombocytopenia syndrome (SFTS) in daily clinical practice. We prepared recombinant SFTS virus-associated protein such as nucleoprotein (NP) and nonstructural S segment (NS) protein. Anti- recombinant NP (rNP) antibody was purified from serum of immunized rabbit with rNP. Double-antigen enzyme-linked immunosorbent assay (ELISA) using rNP detected anti-NP antibody in serum obtained from patients with early onset of SFTS. This assay showed that anti-NP antibody increased in serum within 3 or 4 days after SFTS virus infection. Although immunochromatographic assay (ICA) to detect SFTS virus antigen was also developed using anti-rNP rabbit antibody, the performance of ICA was unstable. In future study, it is necessary to improve the sensitivity of ICA for early diagnosis of SFTS. Double-antigen ELISA may be useful diagnostic tool for SFTS virus infection in a SFTS-endemic area in Japan.
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