| Project/Area Number |
17K15921
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | Yamagata University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | PBC / microRNA / miR-139-5p |
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| Outline of Final Research Achievements |
In the first year, we verified cytokine regulation by miRNA interference in cultured cells. When miR-139-5p interference was performed, TNF-α in the culture supernatant tended to decrease, confirming that miR-139-5p functions as a inflammatory cytokine regulator. In the next fiscal year, we examined the in vivo expression effect of miR-139-5p using PBC model mice. It was confirmed that administration of miR-139-5p inhibitor to the tail vein of NOD.c3c4 mice resulted in a decrease in TNF-α when it was underexpressed in PBC liver. Therefore, miR-139-5p was shown to be involved in the pathogenesis of PBC.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究により難治性肝疾患であるPBCの病態が解明しうる可能性がある。最終的にはmiRNAをターゲットとした創薬開発応用につながることが期待される。根治療法としては肝移植しかなかったPBC治療に大きなブレイクスルーをもたらす成果であったと思われる。
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