| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2019: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
We studied the activation of hepatic stellate cells from the viewpoint of energy metabolism, and found that adenylate isozyme B (AKisoB) is closely related to hepatic stellate cell activation.
Knockdown of AKisoB by microarray analysis increased αSMA by 2.7-fold and Col1A1 by 2.1-fold. When AKisoB was purified by a column attached to protein IIA and evaluated by Western blotting, several bands were confirmed in addition to the AKiso band. The above results proved that AKisoB is involved in the activation of hepatic stellate cells. This study is expected to lead to the discovery of therapeutic agents for improving fibrosis in liver cirrhosis and the analysis of the mechanism of liver fibrosis.
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