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Role of SLPI in the maintenance of intestinal homeostasis

Research Project

Project/Area Number 17K15954
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Gastroenterology
Research InstitutionOita University

Principal Investigator

Akira Sonoda  大分大学, 医学部, 病院特任助教 (40751045)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsSLPI / 腸炎 / TRAF6 / アンピシリン / バンコマイシン / 好中球 / プロテアーゼ阻害 / 腸管バリア機能 / 抗生物質 / 炎症性腸疾患
Outline of Final Research Achievements

We found that LPS stimulation induced SLPI from mouse intestinal epithelial cell line via TRAF6 signal. SLPI has neutrophil elastase inhibitory activity and has been suggested to protect against tissue damage, but its physiological function in intestinal tissues is unknown. Analysis of DSS-induced colitis in SLPI-deficient mice revealed that the pathological condition of mutant mice was exacerbated. It was also found by chance that oral administration of two antibiotics, ampicillin and vancomycin, causes changes in the composition of intestinal microbiota and metabolites, an enlargement of the cecum, a defective intestinal tissue barrier function and fecal occult blood. In this study, we have established a new drug-induced enteritis model and elucidated its pathological mechanism.

Academic Significance and Societal Importance of the Research Achievements

細菌成分によって腸上皮細胞から誘導されるSLPIが、腸管組織における恒常性の維持に重要であることが遺伝子改変マウスの解析から明らかになった。すなわち、SLPIはプロテアーゼ阻害活性により過剰な炎症反応で生じる組織損傷に対して腸管組織を保護していることが示唆された。また、抗生物質投与後に各種腸炎が引きおこされることが知られているが、マウスにアンピシリンとバンコマイシンを投与することで、腸内細菌叢の変化による薬剤起因性腸炎が誘導されることを見出した。新たな腸炎モデルの確立とその病態機序の解明により、今後のヒト腸疾患の予防法・治療法の開発に貢献するものと考えられる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (9 results)

All 2019 2018 2017

All Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (6 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Oral administration of antibiotics results in fecal occult bleeding due to metabolic disorders and defective proliferation of the gut epithelial cell in mice2018

    • Author(s)
      Sonoda Akira、Kamiyama Naganori、Ozaka Sotaro、Gendo Yoshiko、Ozaki Takashi、Hirose Haruna、Noguchi Kaori、Saechue Benjawan、Sachi Nozomi、Sakai Kumiko、Mizukami Kazuhiro、Hidano Shinya、Murakami Kazunari、Kobayashi Takashi
    • Journal Title

      Genes to Cells

      Volume: 23 Issue: 12 Pages: 1043-1055

    • DOI

      10.1111/gtc.12649

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Dysbiosis of the Gut Microbiota on the Inflammatory Background due to Lack of Suppressor of Cytokine Signalling-1 in Mice2018

    • Author(s)
      Gendo Y、Matsumoto T、Kamiyama N、Saechue B、Fukuda C、Dewayani A、Hidano S、Noguchi K、Sonoda A、Ozaki T、Sachi N、Hirose H、Ozaka S、Eshita Y、Mizukami K、Okimoto T、Kodama M、Yoshimatsu T、Nishida H、Daa T、Yamaoka Y、Murakami K、Kobayashi T
    • Journal Title

      Inflammatory Intestinal Diseases

      Volume: 3 Issue: 3 Pages: 145-154

    • DOI

      10.1159/000495462

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Autoimmune sialadenitis is associated with the upregulation of chemokine/chemokine receptor pairs in T cell-specific TRAF6-deficient mice2018

    • Author(s)
      Noguchi Kaori、Kamiyama Naganori、Hidano Shinya、Gendo Yoshiko、Sonoda Akira、Ozaki Takashi、Hirose Haruna、Sachi Nozomi、Saechue Benjawan、Ozaka Sotaro、Eshita Yuki、Mizukami Kazuhiro、Kawano Kenji、Kobayashi Takashi
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 504 Issue: 1 Pages: 245-250

    • DOI

      10.1016/j.bbrc.2018.08.162

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 抗生物質や抗菌ペプチドによる腸内環境の変化が腸内細菌叢に及ぼす影響2019

    • Author(s)
      小坂聡太郎、有木晋平、園田光、Benjawan Saechue、Astri Dewayani、神山長慶、飛彈野真也、水上一弘、村上和成、小林隆志
    • Organizer
      第10回癌・炎症と抗酸化研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 抗生物質起因性大腸炎モデルマウスに対する柴苓湯の保護効果の解明2019

    • Author(s)
      小坂聡太郎、有木晋平、園田光、佐知望美、後藤美月、曽我泰裕、広瀬晴奈、尾崎貴士、Benjawan Saechue、Astri Dewayani、神山長慶、飛彈野真也、水上一弘、村上和成、小林隆志
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] TRAF6 Regulation in TH9 Cell Responses to Tumor Growth2019

    • Author(s)
      Astri Dewayani, Naganori Kamiyama, Shinya Hidano, Takashi Ozaki, Akira Sonoda, Nozomi Sachi, Sotaro Ozaka, Shimpei Ariki, Benjawan Saechue, Takashi Kobayashi
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] アンピシリンとバンコマイシンの投与によってグルタミン代謝と短鎖脂肪酸の発酵が障害され、マウス腸炎が誘発される2017

    • Author(s)
      園田 光, 玄同 淑子, 尾崎 貴士, 佐知 望美, 神山 長慶, 飛弾野 真也, 福田 健介, 首藤 充孝, 小川 竜, 岡本 和久, 水上 一弘, 沖本 忠義, 小林 隆志, 村上 和成
    • Organizer
      第25回日本消化器関連学会週間(JDDW) 第59回日本消化器病学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] A combined administration of ampicillin and vancomycin induces mild colitis with decreased diversity of gut microbiota and perturbation of glutamine and short chain fatty acid metabolisms.2017

    • Author(s)
      A. Sonoda, N. Sachi, Y. Gendo, T. Ozaki, N. Kamiyama, S. Hidano, K. Mizukami, T. Okimoto, K. Murakami, T. Kobayashi
    • Organizer
      25th United European Gastroenterology Week (UEGW)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] アンピシリンとバンコマイシンの投与によってグルタミン代謝と短鎖脂肪酸の発酵が障害され、マウス腸炎が誘発される2017

    • Author(s)
      園田 光, 玄同 淑子, 尾崎 貴士, 佐知 望美, 神山 長慶, 飛弾野 真也, , 村上 和成, 小林 隆志
    • Organizer
      生命科学系学会合同年次大会(ConBio2017)
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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