• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification and analysis of a susceptibility gene for MAC pulmonary disease

Research Project

Project/Area Number 17K16066
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Respiratory organ internal medicine
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

YOSHIDA Mitsunori  国立感染症研究所, ハンセン病研究センター 感染制御部, 主任研究官 (70772630)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords肺MAC症 / 疾患感受性遺伝子 / ケース・コントロール関連解析 / ゲノムワイド関連解析
Outline of Final Research Achievements

Pulmonary Mycobacterium avium complex (MAC) disease is a chronic progressive lung disease. Although epidemiological data indicate potential genetic predisposition, its pathogenesis remains unclear. Conducting a genome-wide association study (GWAS), we previously found a susceptibility gene (hereafter referred to as gene-A) for pulmonary MAC disease. In this study, we confirmed reproducibility of our previous GWAS using independent set of Japanese patients with MAC. In a type II alveolar cell line, expression of gene-A was upregulated on 5 hours after infection with MAC. The gene-A knockdown cell line showed higher bacterial burden than wild-type cell line did on 3 days after infection with MAC. These results suggested that expression of gene-A is required for inhibiting intracellular growth of MAC.

Academic Significance and Societal Importance of the Research Achievements

肺MAC 症患者の急増により、その病態機構の解明と新たな診断方法および治療方法の開発が望まれている。我々の研究により、肺MAC 症の発症と有意に関連する因子が見出され、本研究によりその再現性が確認された。さらに、培養細胞株を使用して、A遺伝子の発現がMAC感染における細胞内殺菌に必要であることを示唆する結果が得られた。将来的に、A 遺伝子が関与するMAC感染・発症防御機構を明らかにすることができれば、得られた知見に基づいた新規治療薬の開発、肺MAC 症のリスク診断やオーダーメイド治療といった新しい診断・治療法開発につながることも十分に期待でき、国民の公衆衛生に大きく貢献することが予想される。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (8 results)

All 2020 2018

All Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Open Access: 5 results) Presentation (1 results)

  • [Journal Article] Complete Genome Sequence of Mycobacterium xenopi JCM15661T, Obtained Using Nanopore and Illumina Sequencing Technologies.2020

    • Author(s)
      Yoshida M, Fukano H, Asakura T, Hisatsune J, Hoshino Y.
    • Journal Title

      Microbiology resource announcements

      Volume: 9 Issue: 10 Pages: 0-0

    • DOI

      10.1128/mra.01583-19

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Discrimination of Mycobacterium leprae and Mycobacterium haemophilum in Clinical Isolates and Specimens by Multiplex PCR Assay and Prediction of Drug Susceptibility2018

    • Author(s)
      Kitaoka Naoya、Fukano Hanako、Yoshida Mitsunori、Miyamoto Yuji、Mori Shuichi、Ishii Norihisa、Ato Manabu、Ohara Naoya、Hoshino Yoshihiko
    • Journal Title

      Journal of Clinical Microbiology

      Volume: 57 Issue: 2 Pages: 1-3

    • DOI

      10.1128/jcm.01760-18

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A rapid and non-pathogenic assay for association of Mycobacterium tuberculosis gyrBA mutations and fluoroquinolone resistance using recombinant Mycobacterium smegmatis2018

    • Author(s)
      Yoshida Mitsunori、Nakata Noboru、Miyamoto Yuji、Fukano Hanako、Ato Manabu、Hoshino Yoshihiko
    • Journal Title

      FEMS Microbiology Letters

      Volume: 365 Issue: 23 Pages: 1-8

    • DOI

      10.1093/femsle/fny266

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mycobacterium shigaense sp. nov., a slow-growing, scotochromogenic species, is a member of the Mycobacterium simiae complex2018

    • Author(s)
      H. Fukano, M. Yoshida, Y. Kazumi, N. Fujiwara, K. Katayama, Y. Ogura, T. Hayashi, Y. Miyamoto, N. Fujimoto, W. Hongsheng, C. Mizumoto, Y. Koizumi, H. Maeda, O. Hiranuma, S. Mitarai, N. Ishii, and Y. Hoshino
    • Journal Title

      Int J Syst Evol Microbiol

      Volume: 68(8) Issue: 8 Pages: 2437-2442

    • DOI

      10.1099/ijsem.0.002845

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Complete Genome Sequence of Mycobacterium shigaense2018

    • Author(s)
      Yoshida Mitsunori、Fukano Hanako、Ogura Yoshitoshi、Kazumi Yuko、Mitarai Satoshi、Hayashi Tetsuya、Hoshino Yoshihiko
    • Journal Title

      Genome Announcements

      Volume: 6 Issue: 25 Pages: 1-3

    • DOI

      10.1128/genomea.00552-18

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Naturally occurring a loss of a giant plasmid from Mycobacterium ulcerans subsp. shinshuense makes it non-pathogenic2018

    • Author(s)
      K. Nakanaga, Y. Ogura, A. Toyoda, M. Yoshida, H. Fukano, N. Fujiwara, Y. Miyamoto, N. Nakata, Y. Kazumi, S. Maeda, T. Ooka, M. Goto, K. Tanigawa, S. Mitarai, K. Suzuki, N. Ishii, M. Ato, T. Hayashi, and Y. Hoshino
    • Journal Title

      Sci Rep

      Volume: 8 Issue: 1 Pages: 8218-8218

    • DOI

      10.1038/s41598-018-26425-1

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Draft Genome Sequence of Mycobacterium montefiorense Isolated from Japanese Black Salamander (Hynobius nigrescens)2018

    • Author(s)
      Fukano Hanako、Yoshida Mitsunori、Shimizu Akane、Iwao Hajime、Katayama Yukie、Omatsu Tsutomu、Mizutani Tetsuya、Kurata Osamu、Wada Shinpei、Hoshino Yoshihiko
    • Journal Title

      Genome Announcements

      Volume: 6 Issue: 21 Pages: 1-3

    • DOI

      10.1128/genomea.00448-18

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] ブルーリ潰瘍の原因菌Mycobacterium ulcerans subsp. shinshuenseの比較ゲノム解析.2018

    • Author(s)
      吉田光範, 星野仁彦
    • Organizer
      第93回日本結核病学会総会
    • Related Report
      2018 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2023-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi