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Building 3D platform of patient iPS cells for drug development

Research Project

Project/Area Number 17K16121
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Neurology
Research InstitutionKyoto University

Principal Investigator

Kondo Takayuki  京都大学, iPS細胞研究所, 特定拠点講師 (80536566)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsiPS細胞 / アルツハイマー病 / 神経細胞 / 分化誘導 / 創薬 / アストロサイト / 三次元培養 / 大脳皮質 / グリア細胞 / オルガノイド / スクリーニング / iPS細胞 / / 疾患モデリング / 三次元 / 脳神経疾患 / 発生・分化 / 再生医学 / 老化
Outline of Final Research Achievements

iPS cells have come to be applied to disease research because of their ability to transform into all types of tissue cells while inheriting the genomic information of patients himself, while maintaining infinite proliferation potential. In particular, a three-dimensional (3D) culture system has been focused on to reproduce an extracellular environment that is similar to the physiological state of the brain, which is the structure surrounded by glial cells. We have successfully differentiated human iPS cells into cortical neurons only in one week. Established neurons can be a substrate for reproducible construction of 3D structures, and we demonstrated their applicability to drug screening platforms. It is expected to be applied to the elucidation of pathology and drug discovery research by constructing 3D brain tissue in the future.

Academic Significance and Societal Importance of the Research Achievements

ヒトiPS細胞から大脳皮質神経細胞を再現性良く短期間で大量に調整する方法を開発した。こうして得られた神経細胞は、疾患病態再現及び創薬プラットフォームへ応用できることも示した。さらにこの神経細胞を集積三次元構造の素子として用いることにより、培養皿の中で3次元ヒト脳組織を再構築し、病態解明と治療法開発につながることを期待される。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (4 results)

All 2020 2018 2017

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (1 results) (of which Invited: 1 results)

  • [Journal Article] Generation of a human induced pluripotent stem cell line derived from a Parkinson's disease patient carrying SNCA duplication2020

    • Author(s)
      Hidefumi Suzuki et al.
    • Journal Title

      Stem Cell Research

      Volume: 45 Pages: 101828-101828

    • DOI

      10.1016/j.scr.2020.101828

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evaluation of Toxic Amyloid β42 Oligomers in Rat Primary Cerebral Cortex Cells and Human iPS-derived Neurons Treated with 10-Me-Aplog-1, a New PKC Activator2020

    • Author(s)
      Murakami Kazuma、Yoshimura Mayuko、Nakagawa Shota、Kume Toshiaki、Kondo Takayuki、Inoue Haruhisa、Irie Kazuhiro
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 21 Issue: 4 Pages: 1179-1179

    • DOI

      10.3390/ijms21041179

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] iPSC-Based Compound Screening and In?Vitro Trials Identify a Synergistic Anti-amyloid β Combination for Alzheimer’s Disease2017

    • Author(s)
      Kondo Takayuki、Imamura Keiko、Funayama Misato、Tsukita Kayoko、Miyake Michiyo、Ohta Akira、Woltjen Knut、Nakagawa Masato、Asada Takashi、Arai Tetsuaki、Kawakatsu Shinobu、Izumi Yuishin、Kaji Ryuji、Iwata Nobuhisa、Inoue Haruhisa
    • Journal Title

      Cell Reports

      Volume: 21 Issue: 8 Pages: 2304-2312

    • DOI

      10.1016/j.celrep.2017.10.109

    • NAID

      120006366743

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] アルツハイマー病患者の神経細胞を用いた化合物スクリーニングと“in vitro trial”2018

    • Author(s)
      近藤孝之
    • Organizer
      第37回日本認知症学会学術集会
    • Related Report
      2018 Research-status Report
    • Invited

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Published: 2017-04-28   Modified: 2021-12-27  

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