• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Clarification of the pathological mechanism of VGCC associated diseases

Research Project

Project/Area Number 17K16128
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Neurology
Research InstitutionYokohama City University

Principal Investigator

Kunii Misako  横浜市立大学, 附属病院, 助教 (80725200)

Research Collaborator Tanaka Fumiaki  
Matsumoto Naomichi  
Doi Hiroshi  
Hashiguchi Syunta  
Ohba Chihiro  
Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
KeywordsCACNA1G / VGCC / てんかん / 発達障害 / チャネル病 / パッチクランプ / T-type VGCC / CaV3.1 / patch-clamp / パッチクランプ法 / 電位依存性カルシウムチャネル / 遺伝性脊髄小脳変性症
Outline of Final Research Achievements

We find three patients with neurodevelopmental disorders harboring de novo CACNA1G mutations.Two of three mutations were reported as childhood-onset cerebellar atrophy (Chemin et al., 2018). To investigate the effect of CaV3.1 mutations on channel function, the WT and each mutant CaV3.1 channel were expressed in HEK-293T cells, and the T-type Ca2+ current was studied with the standard whole-cell patch-clamp technique.I-V curve, activation and steady-state inactivation curves, and tau kinetics were changed in these known mutations.These results were almost the same as previous report. Further, we assessed the electrical resonance of WT and mutant CaV3.1 channels.Now we are preparing for the publishing our report describing clinical phenotypes of the patients with these mutations and findings on channel function obtained by whole-cell voltage-clamp analysis.

Academic Significance and Societal Importance of the Research Achievements

3種類の変異のうち昨年報告された2種類は白人における症例であり,アジア人での報告はまだない.また,同一変異ではあるが臨床所見も一部既報告とは異なる部分がある.CACNA1G変異による疾患の臨床症状の多様さを示しており,さらに未報告の変異1例も含んでおり報告の意義がある.さらに,既報告で行われている一般的な電気生理学的解析のみではなく,共振という電気生理学的現象に着目し,実験を追加した.共振はこれまで各種細胞の特性を明らかにするため着目されてきたが,疾患原因となる変異をもつチャネルでの検討はほとんどなされていない.今後のチャネル病の解明にも有用な手法であり,有用な報告である.

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (9 results)

All 2019 2018 2017

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (5 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Genetic analysis of adult leukoencephalopathy patients using a custom-designed gene panel2018

    • Author(s)
      Kunii M.、Doi H.、Ishii Y.、Ohba C.、Tanaka K.、Tada M.、Fukai R.、Hashiguchi S.、Kishida H.、Ueda N.、Kudo Y.、Kugimoto C.、Nakano T.、Udaka N.、Miyatake S.、Miyake N.、Saitsu H.、Ito Y.、Takahashi K.、Nakamura H.、Tomita-Katsumoto A.、Takeuchi H.、Koyano S.、Matsumoto N.、Tanaka F.
    • Journal Title

      Clinical Genetics

      Volume: - Issue: 2 Pages: 232-238

    • DOI

      10.1111/cge.13371

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cerebellar ataxia-dominant phenotype in patients with ERCC4 mutations2018

    • Author(s)
      Doi H、Koyano S、Miyatake S、Nakajima S、Nakazawa Y、Kunii M、Tomita-Katsumoto A、Oda K、Yamaguchi Y、Fukai R、Ikeda S、Kato R、Ogata K、Kubota S、Hayashi N、Takahashi K、Tada M、Tanaka K、Nakashima M、Tsurusaki Y et al.
    • Journal Title

      Journal of Human Genetics

      Volume: 63 Issue: 4 Pages: 417-423

    • DOI

      10.1038/s10038-017-0408-5

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Matrin 3 Is a Component of Neuronal Cytoplasmic Inclusions of Motor Neurons in Sporadic Amyotrophic Lateral Sclerosis2018

    • Author(s)
      Tada Mikiko、Doi Hiroshi、Koyano Shigeru、Kubota Shun、Fukai Ryoko、Hashiguchi Shunta、Hayashi Noriko、Kawamoto Yuko、Kunii Misako、Tanaka Kenichi、Takahashi Keita、Ogawa Yuki、Iwata Ryo、Yamanaka Shoji、Takeuchi Hideyuki、Tanaka Fumiaki
    • Journal Title

      The American Journal of Pathology

      Volume: 188 Issue: 2 Pages: 507-514

    • DOI

      10.1016/j.ajpath.2017.10.007

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cerebrospinal fluid level of Nogo receptor 1 antagonist lateral olfactory tract usher substance (LOTUS) correlates inversely with the extent of neuroinflammation2018

    • Author(s)
      Takahashi Keita、Takeuchi Hideyuki、Kurihara Yuji、Doi Hiroshi、Kunii Misako、Tanaka Kenichi、Nakamura Haruko、Fukai Ryoko、Tomita-Katsumoto Atsuko、Tada Mikiko、Higashiyama Yuichi、Joki Hideto、Koyano Shigeru、Takei Kohtaro、Tanaka Fumiaki
    • Journal Title

      Journal of Neuroinflammation

      Volume: 15 Issue: 1 Pages: 46-46

    • DOI

      10.1186/s12974-018-1084-x

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] De novo CACNA1G mutations in neurodevelopmental disorders2019

    • Author(s)
      Misako Kunii
    • Organizer
      第60回日本神経学会学術大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] The effects of identified CACNA1G mutations on channel functions in neurodevelopmental disorders2019

    • Author(s)
      Misako Kunii
    • Organizer
      第42回日本神経科学大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Genetic analysis of adult leukoencephalopathy patients using whole exon sequencing2017

    • Author(s)
      M. Kunii, H. Doi, S. Kubota, S. Hashiguchi, N. Hirama, Y. Ogawa, K. Takahashi, K. Tanaka, M. Tada, H. Takeuchi, S. Koyano, N. Matsumoto, F. Tanaka
    • Organizer
      XXIII World Congress of Neurology
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 成人白質脳症の遺伝的背景と画像所見の検討2017

    • Author(s)
      國井美紗子
    • Organizer
      第57回日本核医学会学術総会・第12回アジア・オセアニア核医学会学術会議
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 多発性脳梗塞を呈する血管内リンパ腫で発症したメトトレキサート関連リンパ増殖性疾患の1例2017

    • Author(s)
      國井美紗子,大瀧浩之,浅野徹也,小川有紀,高橋慶太,東山雄一,土井宏,竹内英之,田中章景
    • Organizer
      第29回日本神経免疫学会学術集会
    • Related Report
      2017 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi