| Project/Area Number |
17K16264
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatrics
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| Research Institution | Kumamoto Saishunso National Hospital (2018-2019)
Tottori University (2017) |
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Principal Investigator |
Kurata Hirofumi 独立行政法人国立病院機構熊本再春荘病院(臨床研究部), 臨床研究部, 医師 (00774837)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 発熱 / サイトカイン / 痙攣 / 血液脳関門 / 急性脳症 / 高体温 / リポポリサッカライド / 脳浮腫 / 神経科学 / 脳・神経 |
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| Outline of Final Research Achievements |
Acute encephalopathy (AE) is mainly reported in East Asia and, in most cases, results from pediatric viral infections. At present, Cytokine storm-induced AE is the severest among them. Here, we hypothesized that the induction of systemic inflammation using lipopolysaccharide (LPS) injection followed by hyperthermia (HT) treatment can be used to develop an animal model of AE. Postnatal eight-day-old mouse pups were injected with LPS followed by HT treatment. LPS injection followed by HT treatment increased BBB permeability in the cerebral cortex and the brains of some pups exhibited small ischemic lesions. Our results indicate that a LPS injection followed by HT treatment can produce symptoms of cytokine storm-induced AE. Thus, this mouse model can help to elucidate the pathogenetic mechanisms underlying AE.
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| Academic Significance and Societal Importance of the Research Achievements |
急性脳症は東アジアの小児に多くみられる予後不良な疾患である。有効な治療法は開発されておらず、その原因のひとつに、汎用されるモデル動物が存在しないことがあげられる。我々は、幼若動物に細菌の構成成分を投与し、温熱処置を加えることで脳組織の浮腫を誘発し、急性脳症患者の脳組織に類似する組織所見を得たることができた。このモデル動物は、AEの病態発生メカニズムや治療法の解明に役立つと考えられる。
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