Identification of biomarker for engraftment syndrome after T-cell-rich HLA-haploidentical Hematopoietic Stem Cell Transplantation

• Project/Area Number: 17K16274
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pediatrics
• Research Institution: Fukushima Medical University
• Principal Investigator: OHARA YOSHIHIRO 福島県立医科大学, 医学部, 助教 (80722587)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: 小児T細胞非除去HLA半合致移植 / 生着症候群 / バイオマーカー / 小児腫瘍学

Outline of Final Research Achievements

Luminex was used to measure 48 post-transplant plasma samples for analysis of several inflammatory and anti-inflammatory cytokine. Eleven patients were diagnosed with engraftment syndrome (ES). Comparing the cytokines of the ES-onset group and the non-ES-group, some cytokines on the day 10 and day12 after transplantation showed a significant difference, which could be related to the onset of ES. Besides, some other showed a significant difference between early-onset acute GVHD group and non-GVHD group. Interestingly, the cytokine related to ES or GVHD are different, with suggested that these cytokines could be useful to distinguish ES and aery onset acute GVHD.

Academic Significance and Societal Importance of the Research Achievements

T細胞非除去HLA半合致all-HSCT後のES発症群、非発症群の移植後早期の血漿中サイトカイン動態が異なることが判明した。また同all-HSCT後早期のGVHD発症群と非発症群のサイトカイン動態はES発症群のサイトカイン動態と異なる傾向があり、両者が鑑別可能である可能性が示唆された。今後本研究成果の応用により移植治療の安全性の向上に役立つことが期待される。