Expression of progesterone induced blocking factor (PIBF) in women with severe fetal growth restriction

Research Project

Project/Area Number	17K16310
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Embryonic/Neonatal medicine
Research Institution	Showa University
Principal Investigator	Oba Tomohiro 昭和大学, 医学部, 講師 (60439370)
Project Period (FY)	2017-04-01 – 2020-03-31
Project Status	Completed (Fiscal Year 2019)
Budget Amount *help	¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000) Fiscal Year 2018: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000) Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Keywords	プロゲステロン / 胎児発育不全 / 胎盤機能不全 / 免疫 / PIBF / 母体血清 / 胎盤 / 免疫染色 / トロホブラスト
Outline of Final Research Achievements	A case-control study of pregnant women were conducted. Cases were pregnant women with severe FGR(n=6), whose birth weight were <3% tile. Birth weight of controls were ≧10% tile(n=6). The maternal blood samples were collected at 22-34 weeks of gestation to quentify serum PIBF by Western Blotting. The concentration of band were measured by Image J, and the concentration of FGR were weaker than control. The postpartum placenta samples(n=26) were also collected for immunohistochemical staining to detect PIBF expression in the placenta.In immunohistochemical study, PIBF staining intensities increased in vascular wall and decidua. On intensities of vascular wall, FGR were weaker than control. On intensities of decidua, FGR were same as control. The expression of PIBF might be equivalent in FGR and control. On the maternal and placental circulatuon, PIBF of FGR might be lower than control, that was influential in cytocine balance.
Academic Significance and Societal Importance of the Research Achievements	絨毛細胞が脱落膜に侵入する際に妊娠初期より血清PIBFが低値の症例ではimmunogenic mal-adaptationが生じることで絨毛の浸潤が阻害され胎盤形成が障害されることによりPreeclampsiaやFGRを発症するという仮説をたてた。本研究の最終目標はプロゲステロンを投与し血清PIBFを上昇させることでfeto-maternal interaction でのimmunogenic mal-adaptation の発生を抑制することで胎盤形成を正常化することによりPreclampsiaやFGRの発症を予防することである。