Functional analysis of antigen-specific B cells in systemic sclerosis using micro space
Project/Area Number |
17K16324
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Dermatology
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Research Institution | The University of Tokyo |
Principal Investigator |
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Project Period (FY) |
2017-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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Keywords | 強皮症 / 自己免疫疾患 / B細胞 / サイトカイン / マイクロ・ナノデバイス |
Outline of Final Research Achievements |
Recent advances in immunology have suggested that B cells play a central role in the immune system. B cells are also important in autoimmune diseases, and in particular, self-antigen stimulation via B cell receptors induces activation of autoreactive B cells and cytokine production, resulting in significant involvement in the development and progression of diseases. However, only a few autoreactive B cells exist in the patients, and direct examination of its function such as reactivity to self antigens, cytokine production, and interaction with other cells is technically difficult. Therefore, there are no studies which analyze the role of autoreactive B cells directly. In this study, we examined the function of autoreactive B cells in systemic sclerosis using proprietary techniques and methodologies using microspace.
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Academic Significance and Societal Importance of the Research Achievements |
本研究では新しい測定法を用い、SSc患者より得られた自己反応性B細胞の機能解析を行った。具体的にはガラスマイクロチップ上に作成されたマイクロ空間を用い、topo I抗原特異的B細胞から産生されるサイトカインの測定を行い、モデルマウスへの養子移入を介して自己反応性B細胞の持つ機能を検討した。本研究により、自己反応性B細胞が産生するサイトカインは、自己抗原に対する抗原親和性によって変化することが明らかとなった。これまでブラックボックスであった自己抗原反応性B細胞の機能を直接的に検討したはじめての研究であり、今後の強皮症病態解明に寄与すると考えられる。
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Report
(3 results)
Research Products
(18 results)
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[Journal Article] Rapid alteration of serum interleukin-6 levels may predict the reactivity of i.v. cyclophosphamide pulse therapy in systemic sclerosis-associated interstitial lung disease.2018
Author(s)
Numajiri H, Yoshizaki A, Fukasawa T, Ebata S, Nakamura K, Yamashita T, Saigusa R, Miura S, Hirabayashi M, Yoshizaki A, Sumida H, Asano Y, Kazoe Y, Mawatari K, Kitamori T, Sato S.
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Journal Title
J Dermatol.
Volume: 45
Issue: 10
Pages: 1221-1224
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Serum interleukin-34 levels in patients with systemic sclerosis: Clinical association with interstitial lung disease.2018
Author(s)
Kuzumi A, Yoshizaki A, Toyama S, Fukasawa T, Ebata S, Nakamura K, Yamashita T, Saigusa R, Miura S, Hirabayashi M, Yoshizaki A, Asano Y, Sato S.
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Journal Title
J Dermatol.
Volume: 28
Issue: 10
Pages: 314-319
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] A possible implication of reduced levels of LIF, LIFR, and gp130 in vasculopathy related to systemic sclerosis.2017
Author(s)
sclerosis. Taniguchi T, Miyagawa T, Tamaki Z, Nakamura K, Yamashita T, Saigusa R, Takahashi T, Toyama T, Ichimura Y, Yoshizaki A, Tada Y, Sugaya M, Kadono T, Sato S, Asano Y.
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Journal Title
Arch Dermatol Res.
Volume: 309
Issue: 10
Pages: 833-842
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Fli1 Deficiency Induces CXCL6 Expression in Dermal Fibroblasts and Endothelial Cells, Contributing to the Development of Fibrosis and Vasculopathy in Systemic Sclerosis.2017
Author(s)
Taniguchi T, Asano Y, Nakamura K, Yamashita T, Saigusa R, Ichimura Y, Takahashi T, Toyama T, Yoshizaki A, Sato S.
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Journal Title
J Rheumatol.
Volume: 44
Issue: 8
Pages: 1198-1205
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Epithelial Fli1 deficiency drives systemic autoimmunity and fibrosis: Possible roles in scleroderma.2017
Author(s)
Takehiro Takahashi, Yoshihide Asano, Koji Sugawara, Kouki Nakamura, Takashi Yamashita, Ryosuke Saigusa, Yohei Ichimura, Tetsuo Toyama, Takashi Taniguchi, Kaname Akamata, Shinji Noda, Ayumi Yoshizaki, Daisuke Tsuruta, Maria Trojanowska, and Shinichi Sato
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Journal Title
Journal of Experimental Medicine
Volume: 214
Issue: 4
Pages: 1129-1151
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Single cell analysis revealed that the response to cyclophosphamide therapy is regulated by B cells in systemic sclerosis-associated interstitial lung disease2017
Author(s)
Satoshi Ebata, Ayumi Yoshizaki, Takemichi Fukasawa, Takasgi Yamashita, Shunsuke Miura, Ryosuke Saigusa, Megumi Hirabayashi, Asako Yoshizaki, Kaname Akamata, Yoshihide Asano, Yutaka Kazoe, Kazuma Mawatari, Takehiko Kitamori, Shinichi Sato
Organizer
2017 ACR (81th)/ARHP (52th) Annual Meeting
Related Report
Int'l Joint Research