Measurement analysis of serotonin neurons in central nervous system under real time condition

• Project/Area Number: 17K16387
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Psychiatric science
• Research Institution: Kagoshima University
• Principal Investigator: MORIYA SHUNPEI 鹿児島大学, 医歯学総合研究科, 客員研究員 (60647139)
• Research Collaborator: KUWAKI TOMOYUKI ; YAMASHITA AKIRA
• Project Period (FY): 2017-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 神経活動 / ドーパミン神経 / カルシウムイメージング / ファイバーフォトメトリー / モノアミン神経 / アデノ随伴ウイルス / FLEXシステム / TetOシステム / モノアミンニューロン / 統合失調症 / 神経科学 / 脳・神経

Outline of Final Research Achievements

Dopamine neuron is main neuron in central nervous system and mainly located in ventral tegmental area. The fluctuation of ventral tegmental area dopamine neuronal activity was measured when acute noxious stimuli were given. We utilized a fiber photometry system which has high time resolution by calcium imaging system. We measured specific dopamine neuronal activity using DAT-Cre mice by Cre-loxP system. Fluorescence intensity of G-CaMP6, green fluorescence protein, was analyzed as measurement index. Various stimuli resulted in a rapid increase in the activity of VTA dopamine neuron. We published the paper and presented these findings at a conference.

Academic Significance and Societal Importance of the Research Achievements

我々は、急性の嫌悪刺激が急速に意識清明のマウスの腹側被蓋野におけるドーパミン神経活動を上昇させることを測定結果から明らかにした。測定に非常に高い時間分解能を有しているファイバーフォトメトリーを使用しており、腹側被蓋野のドーパミン神経活動と疼痛や精神疾患への治療方法を関連させて検討する所見として有用である。明確に瞬間的な神経活動を評価できる点でもファイバーフォトメトリーが非常に有用なツールであることを示すものである。

Research Products

• [Journal Article] Acute aversive stimuli rapidly increase the activity of ventral tegmental area dopamine neurons in awake mice. (2018)
  • Author(s): *Moriya S, Yamashita A, Kawashima S, Nishi R, Yamanaka A, Kuwaki T
  • Journal Title: Neuroscience Volume: 386 Pages: 16-23
  • DOI: 10.1016/j.neuroscience.2018.06.027
  • Peer Reviewed / Open Access
• [Journal Article] A variant at 9q34.11 is associated with HLA-DQB1*06:02 negative essential hypersomnia (2018)
  • Author(s): Miyagawa T, Khor SS, Toyoda H, Kanbayashi T, Imanishi A, Sagawa Y, Kotorii N, Kotorii T, Ariyoshi Y, Hashizume Y, Ogi K, Hiejima H, Kamei Y, Hida A, et al.
  • Journal Title: J Hum Genet Volume: 63 Issue: 12 Pages: 1259-1267
  • DOI: 10.1038/s10038-018-0518-8
  • Peer Reviewed / Open Access
• [Presentation] Acute nociceptive stimuli rapidly induces the activity of serotonin and noradrenalin neurons in the brain stem of awake mice (2019)
  • Author(s): Akira Yamashita、Shunpei Moriya、Ryusei Nishi、Yoko Ikoma、Akihiro Yamanaka、Tomoyuki Kuwaki
  • Organizer: 第96回日本生理学会大会
• [Presentation] Acute Aversive Stimuli Rapidly Increase the Activity of Ventral Tegmental Area Dopamine Neurons in Awake Mice (2018)
  • Author(s): Akira Yamashita、Shunpei Moriya、Shigetaka Kawashima、Ryusei Nishi、Akihiro Yamanaka、Tomoyuki Kuwaki
  • Organizer: 第41回日本神経科学大会