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Oncogenesis after hepatic arterial embolization

Research Project

Project/Area Number 17K16441
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Radiation science
Research InstitutionKobe University

Principal Investigator

Ueshima Eisuke  神戸大学, 医学部附属病院, 特定助教 (40645561)

Research Collaborator Takaki Haruyuki  
Kodama Hiroshi  
Nishiofuku Hideyuki  
Hirata Yutaka  
Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsTGF-β1 / HIF-1α / HAE / 動脈塞栓術 / サイトカイン / 低酸素 / 肝細胞癌 / 肝腫瘍新生 / 肝動脈塞栓療法 / Interventional radiology
Outline of Final Research Achievements

Rats bearing N1S1 hepatoma cells underwent HAE. Animals were euthanized at 48h, and liver tissues were harvested. IHC and qPCR were performed to compare the expression of TGF-β1. In vitro experiments with the N1S1 cell line were performed under hypoxic conditions for 48h, and the expression of TGF-β1 and HIF-1α was assessed with western blotting and ELISA. IHC showed that both the TGF-β1 and HIF-1α-positive areas were larger in the HAE group than in the sham group. Similarly, qPCR showed that the mRNA expression levels of TGF-β1 and HIF-1α were higher in the HAE group than those in the sham group. TGF-β1 expression was suppressed when HIF-1α inhibitors were added, and HIF-1α expression was upregulated when exogenous TGF-β1 was added in N1S1 cells.

Academic Significance and Societal Importance of the Research Achievements

肝動脈塞栓術後の癌微小環境において、TGF-β1および HIF-1αが有意に発言していることが分かった。これらは低酸素により発現が促されており、お互い共依存的に上昇することが示された。現在、TGF-β1および HIF-1αの上昇から肝癌の再発・転移に繋がる複数の経路が判明している。これらの一部あるいは複数箇所に作用する阻害薬や抗体を用いることで、肝動脈塞栓術後の再発・転移を抑制し、予後の延長に寄与する可能性があることが示唆された、

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (2 results)

All 2018 2017

All Presentation (2 results)

  • [Presentation] 肝動脈塞栓術がTGF-β発現に与える影響 -ラット肝癌モデルによる検討-2018

    • Author(s)
      上嶋英介、岡田卓也、山口雅人、杉本幸司、村上卓道、西尾福英之、田中利洋、吉川公彦、高木治行、児玉大志、山門亨一郎
    • Organizer
      第65回関西IVR研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 低酸素刺激がサイトカイン産生に与える影響:ラット肝細胞癌株による検討2017

    • Author(s)
      上嶋 英介、岡田卓也、山口雅人、杉本幸司、高木治行、山門亨一郎
    • Organizer
      第63回中部IVR・第64回関西IVR合同研究会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2020-03-30  

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