Analysis of S100 family proteins and exosomal microRNA in duodenal juice as a diagnostic marker of pancreatic ductal adenocarcinoma
| Project/Area Number |
17K16565
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 膵癌 / 十二指腸液 / エクソソーム / 診断マーカー / S100タンパクファミリー / S100タンパク質ファミリー / 膵癌早期発見 |
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| Outline of Final Research Achievements |
S100A2, S100A4, S100A6, and S100A11 concentrations in duodenal juice were measured using a ELISA kit. But the concentrations S100 family protein were not useful for detection of pancreatic ductal adenocarcinoma. We tried to extract exosomes from duodenal juice by ultracentrifugation. We successfully isolated exosomes from duodenal juice. Also we found exosomal RNA (miR-155) might be a biomarker of pancreatic ductal adenocarcinoma.
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| Academic Significance and Societal Importance of the Research Achievements |
膵癌は他癌と比較して極めて予後不良である。この理由として膵癌は早期には症状に乏しく、発見時には進行癌であることが多いことが挙げられる。このため早期発見と効果的なスクリーニング法の開発が求められるが、微小膵癌の段階で診断されるのは極めて稀である。低侵襲に採取可能な十二指腸液中の膵癌早期発見のマーカーを検出することができれば、膵癌を早期に発見することが可能となり、予後改善の足掛かりとなる可能性がある。
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Report
(3 results)
Research Products
(2 results)
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[Journal Article] Pancreatic juice exosomal microRNAs as biomarkers for detection of pancreatic ductal adenocarcinoma2019
Author(s)
Nakamura S, Sadakari Y, Ohtsuka T, Okayama T, Nakashima Y, Gotoh Y, Saeki K, Mori Y, Nakata K, Miyasaka Y, Onishi H, Oda Y, Goggins M, Nakamura M.
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Journal Title
Ann Surg Oncol
Volume: 印刷中
Issue: 7
Pages: 2104-11
DOI
Related Report
Peer Reviewed
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