| Project/Area Number |
17K16597
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cardiovascular surgery
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | エクソソーム / 動脈硬化 / 腹部大動脈瘤 / 大動脈瘤 / マクロファージ / 平滑筋細胞 / 遺伝子 |
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| Outline of Final Research Achievements |
A method for culturing human vascular endothelial cells and human vascular smooth muscle cells was established, and exosomes were extracted. The exosomes extracted by the ultracentrifugation method, coprecipitation method, and immunoassay method were analyzed by a flow cytometer and a dynamic light scattering device. The coprecipitation method was revealed as the most quantitative extraction. ApoE knockout mice were used to stimulate with angiotensin II continuous administration pump, and aortic aneurysm forming model mice could be generated. Human smooth muscle cells were stimulated with angiotensin II, and the expression of Smemb, a proliferative type marker, could be confirmed. The exosomes were extracted from the stimulated smooth muscle cells, and additional experiment was planed.
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| Academic Significance and Societal Importance of the Research Achievements |
動脈硬化に関連する細胞におけるエクソソームに関する研究の足がかりとなった。動脈瘤のモデルマウスを確立し、瘤形成に関わる細胞の表現型を確認し、動脈瘤形成におけるメカニズムを解明する一歩となり得る。より踏み入れたエクソソームの遺伝学的情報を追求していくための基盤ができたと考えられる。
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