Inhibition of proliferation and metastasis of bone soft tissue sarcoma by control of dormancy
| Project/Area Number |
17K16681
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | University of Toyama |
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Principal Investigator |
Suzuki Kayo 富山大学, 学術研究部医学系, 助教 (20456388)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 骨軟部腫瘍 / 肉腫 / 転移 / マウス未分化多形肉腫細胞株 / 高肺転移株 / 低肺転移株 / dormancy / Ki-67 / 骨・軟部腫瘍学 |
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| Outline of Final Research Achievements |
After intramuscular transplantation of HM-RCT, the mice were sacrificed, and the dormancy status was examined by Ki-67 and E2F4 expression in primary and lung metastases. As a result, in the primary lesion, Ki-67 expression was observed at the early stage of tumor formation, peaked by 5-6 weeks when lung metastasis increased. On the other hand, in lung metastases, almost no Ki-67 expression was observed up to 3 weeks, which is the early stage of lung metastasis formation, and E2F4 expression was observed only in a small number of cells, and at 6 weeks when lung metastasis increased. , Ki-67 expression was increased. As a result, Ki-67 positive cells appeared and increased in the primary tumors from the early stage of tumor formation, and the period when tumor growth was high in tumor tissues was maintained as the tumor increased, but when the tumor growth trend stopped, E2F4 The expression cells appeared, and it was suggested that dormancy might occur in a part of the tumor tissue
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| Academic Significance and Societal Importance of the Research Achievements |
軟部肉腫の原発巣および転移巣形成過程における、dormancy存在の可能性を示した。
Dormancy 制御により、肉腫治療の新たな治療戦略の礎になり得る。
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Report
(4 results)
Research Products
(26 results)
