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Development of the injectable biomaterials for vertebral fracture healing

Research Project

Project/Area Number 17K16701
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Orthopaedic surgery
Research InstitutionKitasato University

Principal Investigator

Saito Wataru  北里大学, 医学部, 講師 (60439099)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords人工コラーゲン / コラーゲン結合型成長因子 / 椎体骨折 / 椎体圧迫骨折 / 骨癒合 / 局所投与 / 骨形成タンパク / 再生医学 / 生理活性 / 細菌
Outline of Final Research Achievements

The prevalence of patients with vertebral fracture continues to increase in aging societies, including Japan. Here, we developed an artificial collagen-based bone formation system consisting of poly(Pro-Hyp-Gly)n, which mimics the triple helical conformation of collagen, and basic fibroblast growth factor (bFGF) fused to collagen-binding domain (CBD) of Clostridium histolyticum collagenase. We examined the formation abilitiy of four collagen-binding bFGF consisting consisting of bFGF-s3, bFGF-s2b-s3, bFGF-s3b, and bFGF-s3a-s3b in a mice femoral fracture model. As bFGF-s3a-s3b showed the highest capacity to induce callus formation in the mice fracture model, bFGF-s3a-s3b-loaded artificial collagen gel (ACG) applied the in rat vertebral fracture model and induced bone formation. In addition, combination of bFGF-s3a-s3b and BMP2 promote bone formation. The poly(Pro-Hyp-Gly)n/bFGF-s3a-s3b/BMP-2 construct may therefore have the potential to promote bone formation in clinical settings.

Academic Significance and Societal Importance of the Research Achievements

我が国においても高齢者における骨折は脳血管疾患、高齢による衰弱に次ぐ寝たきり原因の3位であり、椎体骨折後の死亡率は骨折を有しない場合の約4倍の死亡率になるとの報告もある。高齢化の進む我が国の65 歳以上における骨折治療に要する年間医療費の総額は約6000 億円と推測されており、この額は65 歳以上の年間医療費の約4%を占めている。従って高齢者の骨折治癒法の確立は医学的、医療経済的、社会的に重要な意味を持つ。本シーズは、椎体骨折に伴う患者、家族の身体的・精神的苦痛の軽減に加え、経済的な負担軽減、そして医療費の軽減に重要な意義を持つ。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (17 results)

All 2020 2019 2018 2017

All Journal Article (13 results) (of which Peer Reviewed: 13 results,  Open Access: 10 results,  Acknowledgement Compliant: 1 results) Presentation (3 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Risk factors associated with prolonged intensive care unit stay after posterior spinal fusion for Duchenne muscular dystrophy.2020

    • Author(s)
      Saito W, Inoue G, Shirasawa E, Imura T, Nakazawa T, Miyagi M, Uchida K, Matsuda H, Akazawa T, Takaso M.
    • Journal Title

      J Orthop Sci

      Volume: - Issue: 3 Pages: 332-336

    • DOI

      10.1016/j.jos.2020.03.014

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prediction tool for high risk of surgical site infection in spinal surgery.2020

    • Author(s)
      Namba T, Ueno M, Inoue G, Imura T, Saito W, Nakazawa T, Miyagi M, Shirasawa E, Takahashi O, Takaso M
    • Journal Title

      Infect Control Hosp Epidemiol

      Volume: 24 Issue: 7 Pages: 1-6

    • DOI

      10.1017/ice.2020.107

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Occupational Characteristics of Low Back Pain Among Standing Workers in a Japanese Manufacturing Company2020

    • Author(s)
      Inoue G, Uchida K, Miyagi M, Saito W, Nakazawa T, Imura T, Shirasawa E, Akazawa T, Orita S, Inage K, Takaso M, Ohtori S
    • Journal Title

      Workplace Health Saf

      Volume: 68 Issue: 1 Pages: 13-23

    • DOI

      10.1177/2165079919853839

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of single injection of recombinant human bone morphogenetic protein-2-loaded artificial collagen-like peptide in a mouse segmental bone transport model2019

    • Author(s)
      Tazawa R, Minehara H, Matsuura T, Kawamura T, Uchida K, Saito W, Inoue G, Takaso M.
    • Journal Title

      Biomed Res Int

      Volume: - Pages: 1014594-1014594

    • DOI

      10.1155/2019/1014594

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Regression of Retro-odontoid Pseudotumor Using External Orthosis without Atlantoaxial Fusion2019

    • Author(s)
      Nakazawa T, Inoue G, Imura T, Miyagi M, Saito W, Shirasawa E, Kawakubo A, Takaso M
    • Journal Title

      JBJS Case Connect

      Volume: 9 Issue: 3 Pages: e0329-e0329

    • DOI

      10.2106/jbjs.cc.18.00329

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The impact of switching once-weekly teriparatide to denosumab in osteoporosis patients2019

    • Author(s)
      Miyagi M, Fujimaki H, Naruse K, Suto K, Inoue G, Nakazawa T, Imura T, Saito W, Uchida K, Shirasawa E, Takahira N, Takaso M
    • Journal Title

      J Orthop Sci

      Volume: 24 Issue: 1 Pages: 153-158

    • DOI

      10.1016/j.jos.2018.08.001

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Safety and Efficacy of Treatment for Scolios Is Secondary to Spinal Muscular Atrophy Fused to Lumbar 5 Level2018

    • Author(s)
      Takei S, Miyagi M, Saito W, Imura T, Inoue G, Nakazawa T, Shirasawa E, Uchida K, Akazawa T, Takahira N, Takaso M
    • Journal Title

      Spine Surgery and Related Research

      Volume: 2 Issue: 4 Pages: 294-298

    • DOI

      10.22603/ssrr.2017-0093

    • NAID

      130007500798

    • ISSN
      2432-261X
    • Year and Date
      2018-10-26
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] adiographic and Clinical Outcomes From the Use of S2 Alar Screws in Surgery for Adult Spinal Deformity2018

    • Author(s)
      Nakazawa T, Inoue G, Imura T, Miyagi M, Saito W, Shirasawa E, Uchida K, Takahira N, Takaso M
    • Journal Title

      Global Spine J

      Volume: 8 Issue: 7 Pages: 668-675

    • DOI

      10.1177/2192568218762378

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Relationship between Preoperative Echocardiographic Evaluation and Spinal Deformity in Patients with Neuromuscular Scoliosis2018

    • Author(s)
      Saito W, Inoue G, Imura T, Nakazawa T, Miyagi M, Shirasawa E, Uchida K, TakasoM
    • Journal Title

      Spine Surgery and Related Research

      Volume: 2 Issue: 1 Pages: 48-52

    • DOI

      10.22603/ssrr.2017-0018

    • NAID

      130006321439

    • ISSN
      2432-261X
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Perioperative Evaluation of Respiratory Muscle Strength after Scoliosis Correction in Patients with Duchenne Muscular Dystrophy2017

    • Author(s)
      Saito Wataru、Mizuno Kosuke、Inoue Gen、Imura Takayuki、Nakazawa Toshiyuki、Miyagi Masayuki、Shirasawa Eiki、Uchida Kentaro、Takaso Masashi
    • Journal Title

      Asian Spine

      Volume: 11 Issue: 5 Pages: 787-787

    • DOI

      10.4184/asj.2017.11.5.787

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Basic Fibroblast Growth Factor-Anchored Multilayered Mesenchymal Cell Sheets Accelerate Periosteal Bone Formation2017

    • Author(s)
      Uchida K, Inoue G, Matsushita O, Horikawa K, Sekiguchi H, Saito W, Takano S, Fujimaki H, Miyagi M, Takaso M.
    • Journal Title

      Biomed Res Int

      Volume: 4371460 Pages: 1-8

    • DOI

      10.1155/2017/4371460

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Spinal correction in patients with Fukuyama congenital muscular dystrophy2017

    • Author(s)
      Saito Wataru、Namba Takanori、Inoue Gen、Imura Takayuki、Miyagi Masayuki、Nakazawa Toshiyuki、Shirasawa Eiki、Uchida Kentaro、Takaso Masashi
    • Journal Title

      J Orthop Sci

      Volume: 22 Issue: 4 Pages: 658-664

    • DOI

      10.1016/j.jos.2017.02.005

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of Freeze-Dried Allograft Bone with Human Basic Fibroblast Growth Factor Containing a Collagen-Binding Domain From Clostridium Histolyticum Collagenase on Bone Formation After Lumbar Posterolateral Fusion Surgery in Rats2017

    • Author(s)
      Inoue G, Uchida K, Matsushita O, Fujimaki H, Saito W, Miyagi M, Sekiguchi H, Nishi N, Ohtori S, Yogoro M, Takaso M.
    • Journal Title

      Spine

      Volume: 印刷中 Issue: 17 Pages: 995-1001

    • DOI

      10.1097/brs.0000000000002074

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 塩基性線維芽細胞増殖因子と局所硬化デキストランゲルを用いた新規骨形成促進法の有用性2018

    • Author(s)
      庄司真太郎、内田健太郎、関口裕之、井上 玄、齋藤 亘、宮城正行、村田幸佑、川久保 歩、高相晶士
    • Organizer
      第33回日本整形外科学会基礎学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] 小児期側彎症手術における各手術手技段階での血液損失の検討 術中出血低減の試み2017

    • Author(s)
      白澤栄樹、齋藤 亘、宮城正行、井上 玄、井村貴之、中澤俊之、高相晶士
    • Organizer
      第66回日本整形災害外科学会
    • Related Report
      2017 Research-status Report
  • [Presentation] 胸椎に直接浸潤した非小細胞肺癌に対し部分椎体切除を併用し腫瘍を一塊に摘出しえた1例2017

    • Author(s)
      米田昌広、白澤栄樹、井上 玄、齋藤 亘、中澤俊之、井村貴之、宮城正行、高相晶士
    • Organizer
      第66回日本整形災害外科学会
    • Related Report
      2017 Research-status Report
  • [Patent(Industrial Property Rights)] 骨再生用組成物、骨再生用組成物キット、骨再生用部材および骨再生方法2018

    • Inventor(s)
      内田健太郎、齋藤 亘、庄司真太郎、高相晶士 等
    • Industrial Property Rights Holder
      内田健太郎、齋藤 亘、庄司真太郎、高相晶士 等
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Related Report
      2018 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

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