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Analysis of collagen maturation in tendons regulated by mechanosensitive gene Mohawk

Research Project

Project/Area Number 17K16706
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Orthopaedic surgery
Research InstitutionJikei University School of Medicine

Principal Investigator

Kayama Tomohiro  東京慈恵会医科大学, 医学部, 助教 (80793702)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords腱 / 靱帯 / コラーゲン / 運動応答 / 架橋 / Mohawk / メカニカルストレス / メカノセンシング / 再生医学 / 発生
Outline of Final Research Achievements

The research focused on the maturation process of tendons and ligaments. Mechanical stress applied to the animal Achilles tendon has revealed increased collagen thickness. However, this did not directly translate to increased tensile strength. This may be explained by the location of tension experienced by the tendon which have different mechanical properties depending on its function, as demonstrated by the tendon-bone junction and the tendon-muscle junction.

Increased bone-tendon adhesion by the use of a titanium web in anterior cruciate ligament reconstruction, leads to increased strength histologically. Biochemical analysis also confirmed that collagen maturation is improved in these transplanted tendons. Furthermore, the mature collagen cross-links pattern is similar to those of the native tendon. Rigid fixation of the transplanted tendon to bone enables adequate force transmission to the tendon, to promote early maturation.

Academic Significance and Societal Importance of the Research Achievements

腱・靱帯の損傷や断裂は日常生活やスポーツに支障をきたし、保存療法や手術療法を用いても正常な成熟・修復が困難である。本研究は腱・靱帯の主成分であるコラーゲンの成熟が腱と骨の生着強度に関与することを示した。腱が骨に強固に癒合することで、移植腱に物理刺激が伝わり、腱の成熟に影響することが示唆された。本研究は腱・靱帯の成熟・修復に重要な遺伝子とコラーゲン架橋の関係性を明らかにし、今後の保存療法や手術方法、そしてリハビリテーションの改善に貢献するものと考える。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (15 results)

All 2020 2019 2017

All Journal Article (8 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 6 results,  Open Access: 6 results) Presentation (7 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Enhancement of tendon-bone interface healing and graft maturation with cylindrical titanium-web (TW) in a miniature swine anterior cruciate ligament reconstruction model: histological and collagen-based analysis2020

    • Author(s)
      Ryu Keisho、Saito Mitsuru、Kurosaka Daisaburo、Kitasato Seiichiro、Omori Toshiyuki、Hayashi Hiroteru、Kayama Tomohiro、Marumo Keishi
    • Journal Title

      BMC Musculoskeletal Disorders

      Volume: 21 Issue: 1

    • DOI

      10.1186/s12891-020-03199-0

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Pre-operative hemoglobin level and use of sedative-hypnotics are independent risk factors for post-operative delirium following total knee arthroplasty2020

    • Author(s)
      Kijima Eiji、Kayama Tomohiro、Saito Mitsuru、Kurosaka Daisaburo、Ikeda Ryo、Hayashi Hiroteru、Kubota Daisuke、Hyakutake Takashi、Marumo Keishi
    • Journal Title

      BMC Musculoskeletal Disorders

      Volume: 21 Issue: 1 Pages: 279-279

    • DOI

      10.1186/s12891-020-03206-4

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 低侵襲な骨付き膝蓋腱採取法を応用した長方形骨孔の膝前十字靭帯再建術の小経験2020

    • Author(s)
      林大輝、黒坂大三郎、斎藤充、池田亮、窪田大輔、嘉山智大、百武剛志、丸毛啓史
    • Journal Title

      日本関節鏡・膝・スポーツ整形外科学会雑誌

      Volume: 45 Pages: 100-101

    • Related Report
      2019 Annual Research Report
  • [Journal Article] 長方形引き型ダイレーターに夜膝前十字靭帯再再建術の術後成績2020

    • Author(s)
      窪田大輔、林大輝、斎藤充、黒坂大三郎、池田亮、嘉山智大、百武剛志、丸毛啓史
    • Journal Title

      日本関節鏡・膝・スポーツ整形外科学会雑誌

      Volume: 45 Pages: 112-113

    • Related Report
      2019 Annual Research Report
  • [Journal Article] A case of carpal tunnel syndrome caused by giant gouty tophi: The usefulness of DECT for the diagnosis, preoperative planning, and postoperative evaluation of atypical cases.2019

    • Author(s)
      Kazuhiro Maeda, Hiroyuki Chino, Tadashi Tokashiki, Jun Udaka, Yuya Okutsu, Mitsuhito Yukawa, Makoto Mitsuhashi, Naoya Inagaki, Hirofumi Osumi, Yuji Nagamine, Tetsuro Nishizawa, Tomohiro Kayama, Takeshi Fukuda, Kunihiko Fukuda, Hiroya Ojiri, Keishi Marumo
    • Journal Title

      Mod Rheumatol Case Rep.

      Volume: 3 Issue: 2 Pages: 165-171

    • DOI

      10.1080/24725625.2019.1596547

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Positioning the femoral bone socket and the tibial bone tunnel using a rectangular retro-dilator in anterior cruciate ligament reconstruction.2019

    • Author(s)
      Hayashi H, Kurosaka D, Saito M, Ikeda R, Kubota D, Kayama T, Hyakutake T, Marumo K.
    • Journal Title

      PLoS One

      Volume: 14 Issue: 5 Pages: e0215778-e0215778

    • DOI

      10.1371/journal.pone.0215778

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The Regulation of Bone Metabolism and Disorders by Wnt Signaling.2019

    • Author(s)
      Maeda K, Kobayashi Y, Koide M, Uehara S, Okamoto M, Ishihara A, Kayama T, Saito M, Marumo K
    • Journal Title

      Int J Mol Sci

      Volume: 20 Issue: 22 Pages: 5525-5525

    • DOI

      10.3390/ijms20225525

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Mohawk transcription factor regulates homeostasis of the periodontal ligament2017

    • Author(s)
      Koda N, Sato T, Shinohara M, Ichinose S, Ito Y, Nakamichi R, Kayama T, Kataoka K, Suzuki H, Moriyama K, Asahara H
    • Journal Title

      Development

      Volume: 144 Pages: 313-320

    • DOI

      10.1242/dev.135798

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] In vitroとIn vivoでの伸展刺激・運動刺激による腱・靱帯のメカノセンシング機構解析2019

    • Author(s)
      嘉山智大, 森雅樹, 中道亮, 鈴木英嗣, 浅原弘嗣, 斎藤充, 丸毛啓史
    • Organizer
      日本生体電気・物理刺激研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] The role of tendon/ligament-specific transcription factor Mohawk in tendon development, maturation and homeostasis2019

    • Author(s)
      嘉山智大, 森雅樹, 中道亮, 鈴木英嗣, 浅原弘嗣, 斎藤充, 丸毛啓史
    • Organizer
      The 41st Annual meeting of the Royal College of Orthopaedic Surgeons of Thailand
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 低侵襲な骨つき膝蓋腱反射採取法を応用した長方形骨孔の膝前十字靭帯再建術の小経験2019

    • Author(s)
      林大輝、黒坂大三郎、斎藤充、池田亮、窪田大輔、嘉山智大、百武剛志、丸毛啓史
    • Organizer
      日本関節鏡・膝・スポーツ整形外科学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 長方形引き型ダイレーターに夜膝前十字靭帯再再建術の術後成績2019

    • Author(s)
      窪田大輔、林大輝、斎藤充、黒坂大三郎、池田亮、嘉山智大、百武剛志、丸毛啓史
    • Organizer
      日本関節鏡・膝・スポーツ整形外科学会雑誌
    • Related Report
      2019 Annual Research Report
  • [Presentation] In vitroとIn vivoでの伸展刺激・運動刺激による腱・靱帯のメカノセンシング機構解析2019

    • Author(s)
      嘉山智大
    • Organizer
      第46回日本生体電気・物理刺激研究会
    • Related Report
      2018 Research-status Report
  • [Presentation] Mechanoregulatory transcriptional network converges to tendon/ligament-specific Mohawk (Mkx) gene for collagen fiber maturation2017

    • Author(s)
      Tomohiro Kayama, Masaki Mori, Yoshiaki Ito, Ryo Nakamichi, Hidetsugu Suzuki, Takahide Matsushima, Shizuko Ichinose, Mitsuru Saito, Keishi Marumo, Hiroshi Asahara
    • Organizer
      第90回 日本整形外科学会学術総会
    • Related Report
      2017 Research-status Report
  • [Presentation] 腱・靱帯特異的遺伝子Mohawk(MKX)を介した腱細胞の成熟メカニズム2017

    • Author(s)
      嘉山智大、森雅樹、伊藤義晃、中道亮、鈴木英嗣、幸田直己、斎藤充、丸毛啓史、浅原弘嗣
    • Organizer
      第32回 日本整形外科基礎学術集会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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