| Project/Area Number |
17K16893
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Chiba University |
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Principal Investigator |
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| Research Collaborator |
SEKI Naohiko
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| Project Period (FY) |
2017-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 頭頸部扁平上皮癌 / マイクロRNA / インテグリン / 頭頸部癌 |
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| Outline of Final Research Achievements |
A microRNA expression profile was generated using a next-generation sequencer from head and neck squamous cell carcinoma clinical specimens. We extracted microRNA with reduced expression in cancer tissues from profiles and performed functional analysis. As a result, it was found that it functions as a tumor suppressor microRNA. The genes directly regulated by the microRNAs include integrin-related genes, and functional analysis of the genes revealed that they are significantly involved in migration and invasion of head and neck cancer cells. We found that integrin-related genes could be targets for molecular therapy for head and neck squamous cell carcinoma from cell signaling pathway analysis and prognostic analysis.
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| Academic Significance and Societal Importance of the Research Achievements |
申請者が次世代シーケンサーを用いて作成したマイクロRNA発現プロファイルには、今でのアレイ解析では抽出できなかった多くのマイクロRNAが含まれており、プロファイルを基にしたマイクロRNA研究の足掛かりを作ることができた。また、今までは分解され機能しないと考えられてきたパッセンジャー鎖マイクロRNAが癌抑制型マイクロRNAとして機能していることを新たに見出した。癌抑制型マイクロRNAを起点とした分子ネットワークの探索は、頭頸部扁平上皮癌の分子ネットワーク解明に有効であり、新規治療法の開発へ向けて重要な知見を提供する。
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