| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The characteristics and function of vocal fold epithelial cells remains unclear because of the lack of in vitro experiments. In this study, we established the culture methods of the epithelial cells of rat vocal folds to establish an in vitro experimental model. The toluidine blue staining showed multi-layered epithelial structure of the cultured epithelial cell layer and immunohistochemistry revealed the expression of occludin and ZO-1 in the cultured cells. Transepithelial electrical resistance (TER) was measured for the evaluation of epithelial barrier function. Results revealed more than 1000 ohms x cm2 of TER on day 4, passage 1. After exposing the cultured cell layer to acidic-pepsin, TER values promptly decreased and recovered to the baseline within 24 hours.
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