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Investigation of goblet cell hyperplasia mechanism using induced pluripotent stem cells (iPSCs)

Research Project

Project/Area Number 17K16932
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Otorhinolaryngology
Research InstitutionFukushima Medical University

Principal Investigator

Yoshie Susumu  福島県立医科大学, 医学部, 助教 (70705459)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
KeywordsiPS細胞 / 気道上皮 / 杯細胞過形成 / マウスiPS細胞 / ヒトiPS細胞 / 杯細胞過形成モデル / マイクロアレイ解析 / タバコ煙溶液 / マイクロアレイ / 杯細胞 / タバコ煙水溶液 / Muc5ac / 分化誘導
Outline of Final Research Achievements

Airway remodeling provoked by respiratory diseases such as asthma causes goblet cell hyperplasia, submucosal gland hyperplasia, fibrosis under basement membrane, and thickening of smooth muscle. In particular, excessive mucus production due to goblet cell hyperplasia causes severe airway obstruction and destruction of lung tissue, and therefore, elucidation of goblet cell hyperplasia mechanism is needed. The purpose of this study is to elucidate the mechanism of goblet cell hyperplasia by the use of goblet cell hyperplasia model from iPS cells. First, a goblet cell hyperplasia model was generated from mouse iPS cell-derived airway epithelium by the use of cigarette smoking solution. Furthermore, we succeeded in screening of some candidate genes involved in goblet cell hyperplasia by microarray analysis.

Academic Significance and Societal Importance of the Research Achievements

これまでの研究では、主に実験動物によって杯細胞過形成機構の解明が行われてきた。しかし、実験動物を用いた研究では、詳細な分子機序の解明が困難である上に種が異なるためヒトの病態を厳密に模倣することは難しく、病態解明ツールとして適さないと考える。本研究によって、iPS細胞から杯細胞過形成モデルを作製することができれば、実験動物の代替えとなる有効な杯細胞過形成モデルになり、杯細胞過形成機構を細胞レベルから追求していくことが可能になる。さらに、ヒトiPS細胞由来杯細胞過形成モデルを利用し杯細胞過形成機構を解明することができれば、ヒトに対して特異性の高い、新しい治療薬への開発に繋がると考える。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (7 results)

All 2020 2019 2018

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Airway regeneration using iPS cell-derived airway epithelial cells with Cl- channel function2019

    • Author(s)
      Yoshie Susumu, Omori Koichi, and Hazama Akihiro
    • Journal Title

      Channels

      Volume: 13 Pages: 227-34

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Functional characterization of various channel-expressing central airway epithelial cells from mouse induced pluripotent stem cells2019

    • Author(s)
      Yoshie Susumu, Nakamura Ryosuke, Kobayashi Daisuke, Miyake Masao, Omori Koichi, Hazama Akihiro
    • Journal Title

      Journal of Cellular Physiology

      Volume: 印刷中 Issue: 9 Pages: 15951-62

    • DOI

      10.1002/jcp.28254

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] iPS 細胞から CFTR 機能を有する気道上皮組織への分化誘導2020

    • Author(s)
      吉江 進、中村 亮介、小林 大輔、三宅 将生、大森 孝、挾間 章博
    • Organizer
      第97回日本生理学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] In vitro generation of goblet cell hyperplasia model using iPS cells and cigarette smoking solution2019

    • Author(s)
      Yoshie Susumu, Miyake Masao, Hazama Akihiro
    • Organizer
      9th Federation of the Asian and Oceanian Physiological Societies (FAOPS)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Functional characterization of various channel-expressing airway epithelial cells with motile cilia generated from iPS cells2018

    • Author(s)
      Yoshie Susumu, Nakamura Ryosuke, Kobayashi Daisuke, Miyake Masao, Omori Koichi, Hazama Akihiro
    • Organizer
      5th Tissue Engineering and Regenerative Medicine International Society (TERMIS)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 多様なイオンチャネルを発現するiPS細胞由来気道上皮細胞の機能解析2018

    • Author(s)
      吉江進、中村亮介、小林大輔、三宅将生、大森孝一、挾間章博
    • Organizer
      第17回日本再生医療学会総会
    • Related Report
      2017 Research-status Report
  • [Presentation] Functional characterization of various ion channels-expressing airway epithelial cells generated from induced pluripotent stem cells2018

    • Author(s)
      Susumu Yoshie, Ryosuke Nakamura, Daisuke Kobayashi, Masao Miyake, Koichi Omori, Akihiro Hazama
    • Organizer
      第95回日本生理学会大会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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