| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Fibrosis (epithelial-mesenchymal transition) of retinal pigment epithelial cells (RPE) is a finding in advanced stage of age-related macular degeneration. In this study, we investigated the mechanism of EMT in polarized and non-polarized RPE cells. The responses with TGF-beta stimulation, which induces EMT, were found to be different between polarized and nonpolarized RPE. Western blot and immunostaining showed that TGF-beta stimulation reduced the expression of epithelial markers in non-polarized RPE. In addition, the effect was inhibited by protein kinase C (PKC) inhibitor. TGF-beta-induced EMT in nonpolarized RPE might be partly dependent on the PKC pathway.
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