| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
The present study was designed to develop the imaging analysis method for calcification with the combination of proteome and mineralome. In preparation for the development of the analysis method, we determined whether the combination of scanning electron microscopy (SEM) with energy-dispersive X-ray spectroscopy (EDX) and immunostaining would visualize the mineral and protein distribution in the same histological sections. Paraffin sections of fixed rat hindlimbs were processed for SEM-EDX and subsequently for immunofluorescence staining. The localization of calcium, phosphorus, and carbon with type II collagen could be visualized in the same region of calcified growth plate cartilage on the same section. The combination of SEM-EDX and immunostaining is effective for visualizing mineral and protein distribution in the same histological sections. The outcome of the research was reported in Journal of Oral Biosciences 64(4):461-464.
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