| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
The purpose of this study was to investigate osteocytes, phosphorus and autophagy to explore the mechanism of bone senescence. Three types of feed, which have different phosphorus concentration (higher, lower, and normal concentration group, respectively), were fed to mice, since phosphorus is known as an accelerating factor of aging. The senescence characteristics were induced by higher concentration of phosphorus, whereas the production of autophagy associated proteins was altered in both higher and lower concentration groups, which suggest us that the blood level of phosphorus affects autophagy.
Next, osteocyte-like cells obtained from murine femora were cultured under phosphorus controlled medium. The production of autophagy-related proteins was changed in a phosphorus-dose-dependent manner in osteocyte-like cells. Therefore, our findings strongly suggest that autophagy is affected by phosphorus in mouse.
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