Analysis of transcription regulation of Sox9 in salivary gland development
| Project/Area Number |
17K17088
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Showa University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 唾液腺 / Sox9 / 転写因子 / ChIP-seq |
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| Outline of Final Research Achievements |
ox9 regulates many developmental processes. To identify genes that are regulated by Sox9 in salivary gland development, we performed Sox9 ChIP-seq in embryonic salivary gland. A total of 760 regions were detected as Sox9-associated genomic regions. Gene ontology analysis using Genomic Regions Enrichment of Annotations Tool showed that Sox9 peaks were significantly associated with genes related to epithelial tube formation. To confirm the integrity of Sox9 ChIP-seq analysis, we performed de novo motif analysis using whole Sox9 peaks. The consensus Sox dimer motif was identified as the top enriched motif. This motif was mapped to 45% of all Sox9 peaks. Moreover, fifty genes overlapped between those up-regulated according to RNA-seq in embryonic salivary gland. In addition, several overlapping genes were involved in these exocrine glands’ development.
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| Academic Significance and Societal Importance of the Research Achievements |
これまで唾液腺発生研究については分枝形態形成メカニズムについて多くの報告がなされており、初期発生については不明な点が多かった。本研究でSox9は唾液腺においても組織特異的な結合領域を持つことで下流の遺伝子発現を制御し発生過程における細胞分化を制御している可能性が考えられた。Sox9に着目した詳細な解析を進めSox9の作動様式を解明することで唾液腺発生メカニズムの解明が可能となると考えられる。
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Report
(3 results)
Research Products
(13 results)
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[Journal Article] Cdc42 regulates cranial suture morphogenesis and ossification2019
Author(s)
Aizawa Ryo、Yamada Atsushi、Seki Tatsuaki、Tanaka Junichi、Nagahama Ryo、Ikehata Mikiko、Kato Tadashi、Sakashita Akiko、Ogata Hiroaki、Chikazu Daichi、Maki Koutaro、Mishima Kenji、Yamamoto Matsuo、Kamijo Ryutaro
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Journal Title
Biochemical and Biophysical Research Communications
Volume: 512
Issue: 2
Pages: 145-149
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Generation of orthotopically functional salivary gland from embryonic stem cells2018
Author(s)
Tanaka Junichi、Ogawa Miho、Hojo Hironori、Kawashima Yusuke、Mabuchi Yo、Hata Kenji、Nakamura Shiro、Yasuhara Rika、Takamatsu Koki、Irie Tarou、Fukada Toshiyuki、Sakai Takayoshi、Inoue Tomio、Nishimura Riko、Ohara Osamu、Saito Ichiro、Ohba Shinsuke、Tsuji Takashi、Mishima Kenji
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Journal Title
Nature Communications
Volume: 9
Issue: 1
Pages: 4216-4216
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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