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Analysis of transcription regulation of Sox9 in salivary gland development

Research Project

Project/Area Number 17K17088
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Morphological basic dentistry
Research InstitutionShowa University

Principal Investigator

Tanaka Junichi  昭和大学, 歯学部, 助教 (40710166)

Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords唾液腺 / Sox9 / 転写因子 / ChIP-seq
Outline of Final Research Achievements

ox9 regulates many developmental processes. To identify genes that are regulated by Sox9 in salivary gland development, we performed Sox9 ChIP-seq in embryonic salivary gland. A total of 760 regions were detected as Sox9-associated genomic regions. Gene ontology analysis using Genomic Regions Enrichment of Annotations Tool showed that Sox9 peaks were significantly associated with genes related to epithelial tube formation. To confirm the integrity of Sox9 ChIP-seq analysis, we performed de novo motif analysis using whole Sox9 peaks. The consensus Sox dimer motif was identified as the top enriched motif. This motif was mapped to 45% of all Sox9 peaks. Moreover, fifty genes overlapped between those up-regulated according to RNA-seq in embryonic salivary gland. In addition, several overlapping genes were involved in these exocrine glands’ development.

Academic Significance and Societal Importance of the Research Achievements

これまで唾液腺発生研究については分枝形態形成メカニズムについて多くの報告がなされており、初期発生については不明な点が多かった。本研究でSox9は唾液腺においても組織特異的な結合領域を持つことで下流の遺伝子発現を制御し発生過程における細胞分化を制御している可能性が考えられた。Sox9に着目した詳細な解析を進めSox9の作動様式を解明することで唾液腺発生メカニズムの解明が可能となると考えられる。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (13 results)

All 2019 2018 2017

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (10 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results)

  • [Journal Article] Cdc42 regulates cranial suture morphogenesis and ossification2019

    • Author(s)
      Aizawa Ryo、Yamada Atsushi、Seki Tatsuaki、Tanaka Junichi、Nagahama Ryo、Ikehata Mikiko、Kato Tadashi、Sakashita Akiko、Ogata Hiroaki、Chikazu Daichi、Maki Koutaro、Mishima Kenji、Yamamoto Matsuo、Kamijo Ryutaro
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 512 Issue: 2 Pages: 145-149

    • DOI

      10.1016/j.bbrc.2019.02.106

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Generation of orthotopically functional salivary gland from embryonic stem cells2018

    • Author(s)
      Tanaka Junichi、Ogawa Miho、Hojo Hironori、Kawashima Yusuke、Mabuchi Yo、Hata Kenji、Nakamura Shiro、Yasuhara Rika、Takamatsu Koki、Irie Tarou、Fukada Toshiyuki、Sakai Takayoshi、Inoue Tomio、Nishimura Riko、Ohara Osamu、Saito Ichiro、Ohba Shinsuke、Tsuji Takashi、Mishima Kenji
    • Journal Title

      Nature Communications

      Volume: 9 Issue: 1 Pages: 4216-4216

    • DOI

      10.1038/s41467-018-06469-7

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Establishment of mouse gingival junctional epithelial cell line using a bioengineered tooth2018

    • Author(s)
      Seki T, Aizawa R, Tanaka J, Yajima-Himuroa S, Kato M, Tanaka K, Mishima K, Yamamoto M.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 047 Issue: 1 Pages: 167-172

    • DOI

      10.1016/j.bbrc.2018.02.047

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] オルガノイド技術を用いたマウスES細胞由来機能的唾液腺組織の誘導2019

    • Author(s)
      田中準一
    • Organizer
      口腔医科学フロンティア
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] マウスES細胞を用いた3次元唾液腺組織の分化誘導2018

    • Author(s)
      田中準一
    • Organizer
      再生医療学会総会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 転写因子を用いたマウスES細胞由来唾液腺組織の誘導2018

    • Author(s)
      田中準一
    • Organizer
      日本病理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Generation of functional salivary gland organoid from mouse embryonic stem cells2018

    • Author(s)
      田中準一
    • Organizer
      ISSCR
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マウスES細胞を用いた3次元唾液腺組織の分化誘導2018

    • Author(s)
      田中準一
    • Organizer
      第17回日本再生医療学会総会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 唾液腺発生における転写因子の機能解析2017

    • Author(s)
      田中準一
    • Organizer
      第17回抗加齢医学会総会
    • Related Report
      2017 Research-status Report
  • [Presentation] 唾液腺発生における機能的転写因子の同定2017

    • Author(s)
      田中準一
    • Organizer
      第28回日本臨床口腔病理学会総会・学術大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 自己組織化技術を用いたマウスES細胞由来3次元唾液腺組織の誘導2017

    • Author(s)
      田中準一
    • Organizer
      第62回日本唾液腺学会学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] 唾液腺初期発生における転写因子の機能解析2017

    • Author(s)
      田中準一
    • Organizer
      第106回日本病理学会総会
    • Related Report
      2017 Research-status Report
  • [Presentation] SOX9 IS A KEY TRANSCRIPTION FACTOR OF SALIVARY GLAND DEVELOPMENT2017

    • Author(s)
      田中準一
    • Organizer
      International Society for Stem Cell Research 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research

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Published: 2017-04-28   Modified: 2020-03-30  

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