• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The morphological and functional study of the newly discovered hematopoietic structure induced in a severely anemic mouse model.

Research Project

Project/Area Number 17K17089
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Morphological basic dentistry
Research InstitutionNippon Veterinary and Life Science University

Principal Investigator

Otsuka Hirotada  日本獣医生命科学大学, 獣医学部, 講師 (30634844)

Research Collaborator Nakamura Masanori  
Yagi Hideki  
Endo Yasuo  
Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords新規造血構造体 / GCSF / Hisitidine decarboxylase / 窒素含有型ビスホスホネート / 髄外造血 / VCAM-1 / Nカドヘリン / Cxcl12(SDF-1) / 大網 / 重症貧血マウスモデル / 脂肪関連リンパ組織
Outline of Final Research Achievements

The treatment with a combination of NBP with phenylhydrazine (PHZ) induced severely anemia in mouse in association with serum EPO increase. The levels of G-CSF in the serum and HPCs in bone marrow decrease in same time whereas the peripheral blood were increased in the severely anemic mouse model. TER119-positive nucleated cells were observed in the capillary of omentum in severely anemic mice.
These results suggest that the induced hematopoietic structures act as the sites of erythropoiesis and that NBP-induced G-CSF production causes HPCs mobilization, thus the newly discovered hematopoietic structure in this study might be formed.

Academic Significance and Societal Importance of the Research Achievements

本研究においては、窒素含有型ビスホスホネートによる髄外造血誘導の機序を一部解明することが出来た。このことは、これまでに報告されている窒素含有型ビスホスホネートの副作用の解明や予防へとつながる可能性があり、基礎研究のみならず、臨床における適用上の可能性をしめすものである。また、大網については、不明な点が多く、本研究において、造血微小環境を有している可能性やそれ以外のサイトカイン類の発現も示したことは、機能解明へとつなげることが可能である。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (13 results)

All 2019 2018 2017

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 5 results) Presentation (8 results) (of which Invited: 1 results)

  • [Journal Article] Decisive differences in the bone repair processes of the metaphysis and diaphysis in young mice2018

    • Author(s)
      Inoue Satoshi、Otsuka Hirotada、Takito Jiro、Nakamura Masanori
    • Journal Title

      Bone Reports

      Volume: 8 Pages: 1-8

    • DOI

      10.1016/j.bonr.2017.11.003

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Symmetrical retrograde actin flow in the actin fusion structure is involved in osteoclast fusion2017

    • Author(s)
      Takito J, Otsuka H, Inoue S, Kawashima T, Nakamura M
    • Journal Title

      Biol Open

      Volume: 6 Issue: 7 Pages: 1104-1114

    • DOI

      10.1242/bio.025460

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comprehensive genetic exploration of selective tooth agenesis of mandibular incisors by exome sequencing2017

    • Author(s)
      Yamaguchi T, Hosomichi K, Yano K, Kim Y, Nakaoka H, Kimura R, Otsuka H, Nonaka N, Haga S, Takahashi M, Shirota T, Kikkawa Y, Yamada A, Kamijo R, Park SB, Nakamura M, Maki K, Inoue I
    • Journal Title

      Hum Genome Variation

      Volume: 印刷中 Issue: 1 Pages: 17005-17005

    • DOI

      10.1038/hgv.2017.5

    • NAID

      120006891152

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Pulmonary platelet accumulation induced by catecholamines: its nvolvement in lipopolysaccharide-induced anaphylaxis-like shock.2017

    • Author(s)
      Zhiqian Yu, Hiroko Saito, Hirotada Otsuka, Yosuke Shikama, Hiromi Funayama, Mai Sakai, Shigeo Murai, Masanori Nakamura, Takashi Yokochi, Haruhiko Takada, Shunji Sugawara, Yasuo Endo.
    • Journal Title

      International Immunopharmacol

      Volume: 43 Pages: 40-53

    • DOI

      10.1016/j.intimp.2016.11.034

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] New Experimental Anemic Model by Using a Nitrogen- Containing Bisphosphonate2017

    • Author(s)
      Otsuka Hirotada, Nakamura Masanaori
    • Journal Title

      Annals of Nutritional Disorders & Therapy

      Volume: 4 Issue: 2 Pages: 1-3

    • DOI

      10.26420/annnutrdisordther.2017.1043

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] ヒスタミン欠損マウスにおける造血・リンパ性器官の形態学的研究2019

    • Author(s)
      大塚裕忠,角山優輔,遠藤康男,大津浩,中村雅典,添田聡
    • Organizer
      第124回日本解剖学会全国学術集会・総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 窒素含有型ビスホスホネートを用いた重症貧血マウスモデルの確立及び同モデルにおける造血動態の解析2018

    • Author(s)
      大塚裕忠
    • Organizer
      第137回昭和学士会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 髄外造血誘導におけるHistidine decarboxylaseの役割に関する研究2018

    • Author(s)
      大塚裕忠,添田聡,大津浩,中村雅典,尼崎肇
    • Organizer
      第123回日本解剖学会総会・全国学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] 2つの異なる扁平骨における骨修復過程の解析2018

    • Author(s)
      井上知、藤川芳織、大塚裕忠、中村雅典
    • Organizer
      第123回日本解剖学会総会・全国学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] マウス創傷治癒過程における局所的マクロファージ除去の影響2018

    • Author(s)
      藤川芳織、井上知、滝戸次郎、大塚裕忠、中村雅典
    • Organizer
      第123回日本解剖学会総会・全国学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] 新規造血構造体形成時における大網組織造血微小環境の解析2017

    • Author(s)
      大塚裕忠,中村雅典
    • Organizer
      日本比較免疫学会
    • Related Report
      2017 Research-status Report
  • [Presentation] Difference between bone repair process in scapula and calvaria2017

    • Author(s)
      S Inoue, H Otsuka, M Nakamura
    • Organizer
      65th Annual Meeting of the Japanese Association for Dental Research
    • Related Report
      2017 Research-status Report
  • [Presentation] 肩甲骨および頭蓋骨における骨修復過程の比較2017

    • Author(s)
      井上知、大塚裕忠、中村雅典
    • Organizer
      第105回日本解剖学会関東支部学術集会
    • Related Report
      2017 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi