| Project/Area Number |
17K17094
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional basic dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
JIANG Qing 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (00790007)
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| Project Period (FY) |
2017-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 骨代謝学 / Ywhag / Ywhae / survival rate / dwarfism |
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| Outline of Final Research Achievements |
Wild-type and Ywhag-/-, Ywhae-/- samples were collected at E15.5 and E18.5. We stained whole skeletons with alcian blue and alizarin red to identify cartilage and calcified tissues, respectively. Ywhag-/- showed similar level compared with wild-type, while Ywhae-/- embryos showed mild dwarfism, size of Ywhae-/- skeletons was smaller than wild-type.
Due to embryonic lethality of Ywhae-/- mice, wild-type and Ywhag-/-, Ywhae+/- samples were obtained from the mice at 3 weeks and 8 weeks of age. Body weight and bone volume of Ywhag-/- mice and Ywhae+/- mice were lower than that of wild-type mice, especially at 3-6 weeks of age.
The survival rate of Ywhag-/- mice at 8 weeks of age was lower than Ywhag+/+ and Ywhag+/- mice. Survival rate of Ywhae+/- mice was lower than that of wild-type.
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| Academic Significance and Societal Importance of the Research Achievements |
Elucidation of the molecular mechanisms of 14-3-3s will hopefully provide novel insights into the therapy of many diseases, such as osteoporosis, and is useful for new drugs development for osteoporosis and bone regeneration.
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