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An in vitro study of reactive sulfur species for treatment of cartilage diseases

Research Project

Project/Area Number 17K17097
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Functional basic dentistry
Research InstitutionShowa University

Principal Investigator

Hoshino Marie  昭和大学, 歯学部, 助教 (10756464)

Research Collaborator Miyamoto Yoichi  
Kaneko Kotaro  
Nagayama Kazuhiro  
Akiake Takaaki  
Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords軟骨細胞 / 硫化水素 / 活性イオウ分子種 / 呼吸 / 細胞・組織
Outline of Final Research Achievements

Sodium hydrogen sulfide (NaHS), a persulfide generator, accelerated elongation of mouse embryonic tibias in ex-vivo cultures. Histochemical analyses revealed that NaHS expanded width of the proliferating zone in growth plates. While NaHS facilitated proliferation of mouse primary chondrocytes, it did not affect the expression of mRNAs for type II and type X collagens and aggrecan in chondrocytes. In addition, propargylglycine, an inhibitor for cystathionine gamma-lyase, a persulfide-producing enzyme, suppressed both elongation of the tibias and proliferation of chondrocytes. These observations indicate that persulfides promote elongation of bones as a consequence of enhanced proliferation of growth plate chondrocytes.

Academic Significance and Societal Importance of the Research Achievements

軟骨は血管がなく、酸素の供給に乏しい組織である。酸素呼吸以外の方法で増殖に要するエネルギーを獲得していると考えられる。最近、ミトコンドリアにおけるイオウ呼吸の存在が示唆されている。電子供与体として活性イオウ分子分子種を、電子供与体として硫化水素を利用するものである。今回、硫化水素が軟骨細胞の増殖を促進することが明らかとなったことから、酸素の供給に乏しい環境下にある軟骨細胞は、イオウ呼吸を利用している可能性が示唆される。また、今回の成果は、骨伸長の新しい制御方法開発の基礎となるものである。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (6 results)

All 2018 2017 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (1 results) Remarks (3 results)

  • [Journal Article] 8-Nitro-cGMP is a promoter of osteoclast differentiation induced by RANKL2018

    • Author(s)
      Kaneko K.、Miyamoto Y.、Tsukuura R.、Sasa K.、Akaike T.、Fujii S.、Yoshimura K.、Nagayama K.、Hoshino M.、Inoue S.、Maki K.、Baba K.、Chikazu D.、Kamijo R.
    • Journal Title

      Nitric Oxide

      Volume: 72 Pages: 46-51

    • DOI

      10.1016/j.niox.2017.11.006

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 8-Nitro-cGMP promotes bone growth through expansion of growth plate cartilage2017

    • Author(s)
      Hoshino Marie、Kaneko Kotaro、Miyamoto Yoichi、Yoshimura Kentaro、Suzuki Dai、Akaike Takaaki、Sawa Tomohiro、Ida Tomoaki、Fujii Shigemoto、Ihara Hideshi、Tanaka Junichi、Tsukuura Risa、Chikazu Daichi、Mishima Kenji、Baba Kazuyoshi、Kamijo Ryutaro
    • Journal Title

      Free Radic Biol Med

      Volume: 110 Pages: 63-71

    • DOI

      10.1016/j.freeradbiomed.2017.05.022

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 新規シグナル分子8-ニトロ-cGMPは骨の成長を促進した.2018

    • Author(s)
      片岡真理江、宮本洋一、金子児太郎、赤池孝章、近津大地、馬場一美、上條竜太郎
    • Organizer
      第33回日本酸化ストレス学会関東支部会
    • Related Report
      2018 Annual Research Report
  • [Remarks] 研究プロジェクト 昭和大学歯科補綴学講座

    • URL

      http://prostho.showa-u.ac.jp/study_project/

    • Related Report
      2018 Annual Research Report
  • [Remarks] 昭和大学歯学部口腔生化学講座

    • URL

      http://www10.showa-u.ac.jp/~oralbio/

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
  • [Remarks] 昭和大学歯科補綴学講座

    • URL

      http://prostho.showa-u.ac.jp/

    • Related Report
      2017 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2020-03-30  

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