| Project/Area Number |
17K17110
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Showa University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | エクソソーム / miRNA / 扁平上皮癌 / 癌幹細胞 / 実験腫瘍学 |
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| Outline of Final Research Achievements |
In the present study, we aimed at identification of miRNAs on resistance of CDDP in OSCC cells, and the pathological roles oh those. Subsequently, it is shown that miR-629-3p plays an important role not only on CDDP resistance, but also on repression of apoptosis and promotion of cell migration. The results of q-PCR and western blotting analysis that this miRNA inhibits a number of gese expressions. Moreover, in a xenograft mouse experiment, miR-629-3p is shown to be involved in the aquired resistance of CDDP.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究によって、miR-629-3pは口腔癌細胞においてCDDPの耐性獲得に関与し、アポトーシスの抑制と細胞遊走能の促進に関与していることが明らかとなった。さらに、本研究結果はCDDP耐性の克服に貢献するだけではなく、術後のQOLの改善にもつながることが期待される。したがって本研究結果は、口腔外科領域における癌治療の成績および患者の予後の飛躍的な向上に重要な役割を果たすことが可能と示唆される。
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