| Project/Area Number |
17K17161
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Prosthodontics/ Dental materials science and
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| Research Institution | Niigata University |
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Principal Investigator |
EGUCHI Kaori 新潟大学, 医歯学総合病院, 助教 (10779614)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | IGFBP-3 / 骨代謝 / 骨形成 / BMP-2シグナル / 骨増生 / IGFBP3 / BMP2 |
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| Outline of Final Research Achievements |
In this study, we focused on the possibility that insulin-like growth factor binding protein-3 (IGFBP-3) has a new IGF-independent function in bone tissue, and IGFBP-3 may play a part in the regulation of bone metabolism. The aim of this project was to analyze the effect of IGFBP-3 on the differentiation of mesenchymal stem cells into osteoblasts. The addition of IGFBP-3 in the osteoblast culture system under certain conditions has decreased the gene expression level of the osteoblast differentiation marker, lowered ALP activity, and suppressed calcification. Furthermore, it was suggested that IGFBP-3 might suppress osteoblast differentiation and maturation by inhibiting BMP-2 signaling.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究ではIGFBP-3が間葉系幹細胞の骨芽細胞分化および成熟に対して抑制的な影響を及ぼすことを明らかにした。また、その抑制的作用はBMP-2シグナルを介してもたらされている可能性が示唆された。IGFBP-3による骨芽細胞分化制御は新たな知見である。IGFBP-3の機能を明らかにし、骨リモデリング機構に関わる分子の機能的意義を解明することは、人為的な骨代謝制御を可能とする新たな骨増生法の開発への応用が期待される。
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