Elucidation of the mechanism of sleep masticatory muscle activity using iPS cell-derived 5-HT receptor-expressing neurons.
| Project/Area Number |
17K17191
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Prosthodontics/ Dental materials science and
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| Research Institution | Showa University |
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Principal Investigator |
Tozawa Yurie 昭和大学, 歯学部, 兼任講師 (70783356)
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| Project Period (FY) |
2017-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 睡眠時ブラキシズム / ヒトiPS細胞 / セロトニン2A受容体 / rs6313 / レポーターレンチウイルス / ホールセルパッチクランプ解析 / 一塩基多型 / 疾患特異的iPS細胞 / SNP / iPS細胞 / 5-HT2A受容体 / 遺伝子多型 |
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| Outline of Final Research Achievements |
The pathogenic mechanism of SB (sleep bruxism) is still unclear. Our previous studies have clarified the relationship between SB and the single nucleotide polymorphism (SNP) of the serotonin 2A receptor gene (HTR2A). In this study, we successfully constructed a functional analysis system specific to human iPS cell-derived HTR2A-positive neurons in order to perform neurophysiological analysis. In the future, if this analysis system can be applied to detect SB-specific electrophysiological parameters, it is expected to lead to the elucidation of the pathogenic mechanism and the development of therapeutic agents.
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| Academic Significance and Societal Importance of the Research Achievements |
睡眠時ブラキシズム(SB)は睡眠関連運動障害の一種であり, その過大な咬合力は患者のQOLを著しく損なう可能性があり, 歯科治療の予後を考える上でSBのマネジメントは非常に重要であると言える．本研究にて構築された解析システムは，ヒトiPS細胞の技術を応用することで．これまで直接的な検証は困難とされていた，脳内に存在するターゲットに対してのアプローチを可能とした．また, 研究のターゲットとしているセロトニン2A受容体は様々な神経系疾患との関連も報告されていることから, 本研究成果は歯科領域のみならず, 医科領域への波及効果も大きいと考えられる.
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Report
(6 results)
Research Products
(9 results)
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[Journal Article] Generation of neural cells using iPSCs from sleep bruxism patients with 5-HT2A polymorphism2017
Author(s)
Hoashi Y, Okamoto S, Abe Y, Matsumoto T, Tanaka J, Yoshida Y, Imaizumi K, Mishima K, Akamatsu W, Okano H, Baba K
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Journal Title
Journal of Prosthodontic Research
Volume: 61
Issue: 3
Pages: 242-250
DOI
NAID
ISSN
1883-1958, 1883-9207
Related Report
Peer Reviewed
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[Presentation] 睡眠時ブラキシズム特異的iPS細胞由来GABA作動性神経細胞の電気生理学的評価2019
Author(s)
中井健人, 安部友佳, 帆足有理恵, Avijite Kumer Sarkar, 小溪啓介, 松本貴志, 安原理佳, 美島健二, 中村史朗, 井上富雄, 志賀孝宏, 赤松和土, 馬場一美
Organizer
日本補綴歯科学会 第128回学術大会
Related Report
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[Presentation] Patch-clamp recordings of neurons induced from sleep bruxism patient-specific iPSCs2019
Author(s)
Nakai K, Abe Y, Hoashi Y, Nakamura S, Shiga T, Avijite K S, Yasuhara R, Matsumoto T, Kotani K, Inoue T, Mishima K, Akamatsu W, Baba K
Organizer
97th General Session & Exhibition of the IADR
Related Report
Int'l Joint Research
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[Presentation] ELECTROPHYSIOLOGICAL RECORDINGS OF NEURONS DERIVED FROM SLEEP BRUXISM PATIENT-SPECIFIC IPSCS2019
Author(s)
Nakai K, Abe Y, Shiga T, Hoashi Y, Nakamura S, Avijite K S, Yasuhara R, Matsumoto T, Kotani K, Inoue T, Mishima K, Akamatsu W, Baba K
Organizer
ISSCR 2019 Annual Meeting
Related Report
Int'l Joint Research
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[Presentation] In vitro disease modeling for sleep bruxism using induced pluripotent stem cells2018
Author(s)
Tozawa Y, Nakai K, Yoneima K, Tanaka J, Matsumoto T, Abe Y, Imaizumi K, Mishima K, AKamatsu W, Okano H, Baba K
Organizer
The 65 th Annual Meeting of Japanese Association for Dental Research
Related Report
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