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Molecular analysis of ameloblastoma exacerbation factor for development of new therapeutic agents

Research Project

Project/Area Number 17K17286
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Surgical dentistry
Research InstitutionIwate Medical University

Principal Investigator

Ishikawa Taichi  岩手医科大学, 歯学部, 助教 (10569247)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsエナメル上皮腫 / 歯周病原細菌 / 酪酸 / Butyric acid / Butyric Acid / 微生物
Outline of Final Research Achievements

It was suggested that Laminin332 is involved in subepithelial infiltration of ameloblastoma.
In addition, the possibility was considered that butyric acid produced by oral bacteria, especially periodontopathic bacteria, stimulates the production of EGF and TGFβ1 from ameloblastoma, and these cytokines increase the expression of Laminin332 involved in invasion by acting on autocrine ameloblastoma.
Therefore, it was suggested that local control of butyric acid-producing bacteria is very important in the infiltration (malignant transformation) of ameloblastoma.

Academic Significance and Societal Importance of the Research Achievements

エナメル上皮腫の顎骨内の侵襲的な増大や,まれに見られる悪性化・転移には,増悪因子としてLaminin等の発現や,サイトカインの関与が示唆されてきた.しかし,腫瘍の部位により細胞に多様性のある本疾患の悪性化に関わるメカニズムについては不明な点が多かった.さらに,その増悪には歯周病原細菌の関与が疑われるものの,それらの影響はこれまで全く検討されていなかった.本研究では,同一のエナメル上皮腫組織から樹立された新たな3種の細胞株を用い,細胞接着因子,サイトカインに加え,細菌由来因子も含めた本疾患の増悪に関するメカニズムを分子生物学的観点から明らかにしたことで,新規治療薬開発の一助となると考えられる.

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (11 results)

All 2020 2019 2018 2017

All Journal Article (6 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results,  Open Access: 5 results) Presentation (5 results)

  • [Journal Article] Effects of butyric acid, a bacterial metabolite, on the migration of ameloblastoma mediated by laminin 3322020

    • Author(s)
      Ishikawa T, Terashima J, Shimoyama Y, Ohashi Y, Mikami T, Takeda Y, Sasaki M
    • Journal Title

      Journal of Oral Science (accepted)

      Volume: accepted

    • NAID

      130007908589

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Identification of HACEK Group Bacteria from Blood Samples of Patients with Infective Endocarditis by PCR-RFLP of the 16s rRNA Gene2018

    • Author(s)
      Sasaki Minoru、Ishikawa Taichi、Shimoyama Yu、Kodama Yoshitoyo、Tajika Shihoko、Kimura Shigenobu
    • Journal Title

      Research Journal of Microbiology

      Volume: 13 Issue: 2 Pages: 93-99

    • DOI

      10.3923/jm.2018.93.99

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The fibronectin-binding protein homologue Fbp62 of Streptococcus anginosus is a potent virulence factor2018

    • Author(s)
      Kodama Y, Ishikawa T, Shimoyama Y, Sasaki D, Kimura S, Sasaki M.
    • Journal Title

      Microbiol. Immunol.

      Volume: 62 Issue: 10 Pages: 624-634

    • DOI

      10.1111/1348-0421.12646

    • NAID

      40021702616

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Distribution of dipeptidyl peptidase (DPP) 4, DPP5, DPP7, and DPP11 in human oral microbiota ? potent biomarkers indicating presence of periodontopathic bacteria2018

    • Author(s)
      Ohara-Nemoto Yuko、Shimoyama Yu、Nakasato Manami、Nishimata Haruka、Ishikawa Taichi、Sasaki Minoru、Kimura Shigenobu、Nemoto Takayuki K
    • Journal Title

      FEMS Microbiology Letters

      Volume: 365 Pages: 1-7

    • DOI

      10.1093/femsle/fny221

    • NAID

      120006987966

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Porphyromonas gingivalis is highly sensitive to inhibitors of a proton-pumping ATPase.2018

    • Author(s)
      Mizuki Sekiya, Yu Shimoyama, Taichi Ishikawa, Minoru Sasaki, Masamitsu Futai, Mayumi Nakanishi-Matsui.
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 498 Issue: 4 Pages: 837-841

    • DOI

      10.1016/j.bbrc.2018.03.066

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Aciduricity and acid tolerance mechanisms of Streptococcus anginosus.2018

    • Author(s)
      Minoru Sasaki, Yoshitoyo Kodama, Yu Shimoyama, Taichi Ishikawa, Shigenobu Kimura.
    • Journal Title

      J Gen. Appl. Microbiol. (in press)

      Volume: 印刷中

    • NAID

      130007494295

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Butyric acid (Periodontopathic bacterial metabolite)effects on aggravation of ameloblastoma2020

    • Author(s)
      Ishikawa T, Shimoyama Y, Kodama Y, Sasaki M
    • Organizer
      第93回日本細菌学会総会
    • Related Report
      2019 Annual Research Report
  • [Presentation] A periodontopathic bacterial metabolite effects on exacerbation of ameloblastoma2019

    • Author(s)
      Ishikawa T, Shimoyama Y, Kodama Y, Sasaki M
    • Organizer
      第61回歯科基礎医学会学術大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Influences of the butyric acid produced by periodontopatheic bacteria on ameloblastoma.2018

    • Author(s)
      石河太知、下山 佑、古玉芳豊、木村重信、佐々木実
    • Organizer
      第91回日本細菌学会総会
    • Related Report
      2018 Research-status Report 2017 Research-status Report
  • [Presentation] Streptococcus anginosus 菌体外タンパク質抗原の炎症応答誘導2018

    • Author(s)
      下山 佑,石河太知,古玉芳豊,木村重信,佐々木実
    • Organizer
      第60回歯科基礎医学会学術大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Streptococcus intermediusの上皮細胞付着能および膿瘍形成能に及ぼすフィブロネクチン結合タンパク質の役割2017

    • Author(s)
      古玉芳豊,下山 佑,石河太知,木村重信,佐々木実
    • Organizer
      第71回日本細菌学会東北支部総会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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