Scx regulates differentiation of PDL cells into osteoblasts under tensile force loading

Research Project

Project/Area Number	17K17305
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Orthodontics/Pediatric dentistry
Research Institution	Tohoku University
Principal Investigator	Kawatsu Masayoshi 東北大学, 歯学研究科, 大学院非常勤講師 (70712925)
Project Period (FY)	2017-04-01 – 2020-03-31
Project Status	Completed (Fiscal Year 2019)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords	scleraxis / 歯根膜細胞 / 機械的刺激 / Ｓｃｌｅｒａｘｉｓ / メカノトランスダクション / Scleraxis
Outline of Final Research Achievements	I examined effect of Scx on tensile force-induced osteoblast differentiation and its underlying mechanisms. In the mouse experimental tooth movement model, expression of Scx,TGF-β1, p-Smad3 increased on the tension side PDL. I loaded tensile force onto cultured PDL cells. The in vitro data showed TGF signaling regulated tensile force-induced Scx expression. Scx knockdown in PDL cells upregulated ALP expression, while inhibited ephrin A2 expression.
Academic Significance and Societal Importance of the Research Achievements	本研究により、メカニカルストレスが負荷された環境におけるScxによるPDL細胞の骨芽細胞分化の抑制的制御と、その分子メカニズムが明らかになった。これらの成果は、歯根膜細胞シートを用いた歯周組織再生治療方法の開発へとつながる基盤的知見となり得る。