Scx regulates differentiation of PDL cells into osteoblasts under tensile force loading
| Project/Area Number |
17K17305
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | scleraxis / 歯根膜細胞 / 機械的刺激 / Ｓｃｌｅｒａｘｉｓ / メカノトランスダクション / Scleraxis |
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| Outline of Final Research Achievements |
I examined effect of Scx on tensile force-induced osteoblast differentiation and its underlying mechanisms. In the mouse experimental tooth movement model, expression of Scx,TGF-β1, p-Smad3 increased on the tension side PDL. I loaded tensile force onto cultured PDL cells. The in vitro data showed TGF signaling regulated tensile force-induced Scx expression. Scx knockdown in PDL cells upregulated ALP expression, while inhibited ephrin A2 expression.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究により、メカニカルストレスが負荷された環境におけるScxによるPDL細胞の骨芽細胞分化の抑制的制御と、その分子メカニズムが明らかになった。これらの成果は、歯根膜細胞シートを用いた歯周組織再生治療方法の開発へとつながる基盤的知見となり得る。
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Report
(4 results)
Research Products
(2 results)
