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A basic study of a new periodontal regenerative therapy with nanoparticles of hydroxyapatite and Low Intensity Pulsed Ultrasound

Research Project

Project/Area Number 17K17345
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Periodontology
Research InstitutionTohoku University

Principal Investigator

SAKISAKA Yukihiko  東北大学, 大学病院, 医員 (10760457)

Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords歯科 / 再生医療 / シグナル伝達 / 細胞・組織 / メカニカルストレス / 歯学 / 歯周再生医学 / ハイドロキシアパタイト / LIPUS
Outline of Final Research Achievements

Nano-sized hydroxyapatite (nano-HA) is known as a bone graft material, and low intensity pulsed ultrasound (LIPUS) is used in clinical applications for fracture treatment. In this study, we evaluated the synergistic effect between nano-HA and LIPUS for a new periodontal regenerative technique.
Nano-HA induced osteopontin gene expression, osteogenic marker, in murine dental follicle cell line (SVF4), murine pluripotent mesenchymal cell line and murine preosteoblast cell line. LIPUS stimulated canonical Wnt signaling in SVF4.
We also assessed the effects of them in immune cells. LIPUS induced gene expression of osteoprotegerin, also known as osteoclastogenesis inhibitory factor, in murine macrophage-like cell line.

Academic Significance and Societal Importance of the Research Achievements

骨置換性にすぐれ、その表面性状から薬物の徐放能を有するドラッグデリバリー材料であると同時に遺伝子デリバリーシステムとしての有用な材料であるnano-HAはそれ自体が硬組織形成を誘導することに加え、LIPUSが前セメント芽細胞と考えられている歯小嚢細胞に対して古典的Wntシグナルを介して硬組織形成細胞への分化を誘導することが示唆された。またLIPUSは創傷治癒過程において司令塔の役割を果たすマクロファージに対しても破骨細胞の分化誘導を抑制する作用が示されたことから、歯周組織の再生に対しても有用である可能性が示された。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (13 results)

All 2018 2017

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (10 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Cyclic Stretch Negatively Regulates IL-1β Secretion Through the Inhibition of NLRP3 Inflammasome Activation by Attenuating the AMP Kinase Pathway2018

    • Author(s)
      Maruyama K, Sakisaka Y, Suto M, Tada H, Nakamura T, Yamada S, Nemoto E.
    • Journal Title

      Front. Physiol.

      Volume: 9 Pages: 802-802

    • DOI

      10.3389/fphys.2018.00802

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Extracellular calcium increases fibroblast growth factor 2 gene expression via extracellular signal-regulated kinase 1/2 and protein kinase A signaling in mouse dental papilla cells2018

    • Author(s)
      Kanaya Sousuke、Xiao Binlu、Sakisaka Yukihiko、Suto Mizuki、Maruyama Kentaro、Saito Masahiro、Nemoto Eiji
    • Journal Title

      Journal of Applied Oral Science

      Volume: 26 Issue: 0 Pages: 231-241

    • DOI

      10.1590/1678-7757-2017-0231

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The effect of Berberine on cell differentiation and proliferation in human periodontal ligament cells2018

    • Author(s)
      Ikeno Shuko、Nemoto Eiji、Kanaya Sousuke、Suto Mizuki、Sakisaka Yukihiko、Shimauchi Hidetoshi、Yamada Satoru
    • Journal Title

      Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology)

      Volume: 60 Issue: 1 Pages: 13-25

    • DOI

      10.2329/perio.60.13

    • NAID

      130006594281

    • ISSN
      0385-0110, 1880-408X
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Serial cultivation of osteogenic cells by automatic transfer between culture substrate of microperforated titanium membrane2018

    • Author(s)
      向阪幸彦、丸山顕太郎、張井玉、石幡浩志、根本英二、佐々木 啓一、初澤毅、山田聡
    • Organizer
      第3回生体医歯工学共同研究拠点国際シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] Cyclic stretch inhibits IL-1β secretion by attenuating NLRP3 inflammasome activation2018

    • Author(s)
      Eiji Nemoto, Kentaro Maruyama, Yukihiko Sakisaka, Mizuki Suto, Hiroyuki Tada, Takashi Nakamura, Satoru Yamada
    • Organizer
      The 104th Annual Meeting of the American Academy of Periodontology in collaboration with the Canadian Academy of Periodontology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] LIPUS inhibits IL-β secretion through NF-κB signaling pathway in macrophages2018

    • Author(s)
      Mizuki Suto, Eiji Nemoto, Kentaro Maruyama, Yukihiko Sakisaka, Satoru Yamada
    • Organizer
      The 104th Annual Meeting of the American Academy of Periodontology in collaboration with the Canadian Academy of Periodontology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Application of microperforated titanium mesh for tissue engineered regeneration2018

    • Author(s)
      Yukihiko Sakisaka, Kentaro Maruyama, Jingyu Zhang, Hiroshi Ishihata, Eiji Nemoto, Keiichi Sasaki, Takeshi Hatsuzawa, Satoru Yamada
    • Organizer
      The 104th Annual Meeting of the American Academy of Periodontology in collaboration with the Canadian Academy of Periodontology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 新規GBR用純チタン製メンブレンの有用性ついて:βTCPを用いたin vivoでの評価2018

    • Author(s)
      須藤瑞樹、石幡浩志、向阪幸彦、山田聡、 長谷川博
    • Organizer
      第61回秋季日本歯周病学会学術大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 骨補填材を用いたGBRにおける純チタン製メンブレンの有効性ついて2018

    • Author(s)
      石幡浩志、須藤瑞樹、向阪幸彦、小松秀裕、丸山顕太郎、山田聡、 長谷川博
    • Organizer
      第61回秋季日本歯周病学会学術大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] in vivoでの新規GBR用純チタン製メンブレンの有効性ついて2018

    • Author(s)
      向阪幸彦、石幡浩志、須藤瑞樹、小松秀裕、丸山顕太郎、山田聡、長谷川博
    • Organizer
      第61回秋季日本歯周病学会学術大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] チタン製メンブレンを用いた新規細胞培養法の開発2018

    • Author(s)
      向阪幸彦、丸山顕太郎、張井玉、石幡浩志、根本英二、佐々木啓一、初澤毅、山田聡
    • Organizer
      平成29年度生体医歯工学共同研究拠点成果報告会(横浜)
    • Related Report
      2017 Research-status Report
  • [Presentation] 周期的伸展刺激はマクロファージNLRP3インフラマソーム活性を抑制する2017

    • Author(s)
      丸山顕太郎、須藤瑞樹、向阪幸彦、金谷総介、根本英二
    • Organizer
      日本炎症・再生医学会(大阪)
    • Related Report
      2017 Research-status Report
  • [Presentation] Application of microperforated titanium mesh for the tissue engineered regeneration therapy2017

    • Author(s)
      向阪幸彦、丸山顕太郎、張井玉、石幡浩志、根本英二、佐々木啓一、初澤毅、山田聡
    • Organizer
      第2回生体医歯工学共同研究拠点国際シンポジウム(東京)
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2020-03-30  

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