| Project/Area Number |
17K17359
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Periodontology
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| Research Institution | Showa University |
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Principal Investigator |
Himuro Sara 昭和大学, 歯学部, 助教 (90736513)
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| Project Period (FY) |
2017-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 接合上皮 |
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| Outline of Final Research Achievements |
We have succeeded in isolating JE cells expressing green fluorescent protein (GFP) by using a bioengineered tooth technique in mice. The gene expressions of GFP-positive JE cells, isolated from around the erupted bioengineered teeth using flow cytometry, were analyzed by RNA sequencing. GFP-positive cells derived from the bioengineered tooth germs showed similar gene expression patterns to primary JE cells. The isolated GFP-positive JE cells were immortalized by transducing the simian virus 40 large T antigen using lentiviral vectors. The established GFP-positive JE cells maintained proliferative activity for more than 20 passages, and did not show cellular senescence as demonstrated by β-galactosidase assay. These cells also expressed similar gene expression patterns to primary JE cells. The established cell lines may prove useful for future investigation of JE characteristics in vitro.
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| Academic Significance and Societal Importance of the Research Achievements |
接合上皮は歯周病の起始点となる重要な組織でありながら、今まで、接合上皮細胞株が樹立されていなかった。今回、天然の接合上皮株と遺伝子発現パターンが酷似した不死化GFP陽性接合上皮株を樹立したことにより、in vitroの接合上皮細胞の研究が進むことが予想され、歯周病発症の機序の解明に繋がると考える。
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