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RNA-seq based study on plant-fungi interaction in melon plant

Research Project

Project/Area Number 17K17626
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Horticultural science
Science in genetics and breeding
Research InstitutionNational Agriculture and Food Research Organization (2018)
University of Tsukuba (2017)

Principal Investigator

Yano Ryoichi  国立研究開発法人農業・食品産業技術総合研究機構, 高度解析センター, 主任研究員 (00443044)

Research Collaborator EZURA Hiroshi  
NONAKA Satoko  
FUJIUCHI Naomichi  
Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Keywordsゲノム科学 / トランスクリプトーム解析 / 菌叢解析 / フィールドオミックス / バイオインフォマティクス / トランスクリプトーム情報 / ウリ科作物 / 温室栽培 / トランスクリプトーム / マイクロバイオーム / メタゲノム / マスクメロン / ハウス栽培 / 園芸学 / ゲノム / 遺伝子
Outline of Final Research Achievements

Although melon is an important source of vitamins and minerals for human, some cultivars (e.g. Japanese Earl’s favorite series melons) are known to produce valuable fruits with rich flavor and sweet taste. However, some of the high-grade melon cultivars are sensitive to fungal diseases such as powdery mildew which sometimes causes severe economic loss in agriculture. In addition to powdery mildew, many fungi are likely to be present on the surface of the melon plants grown under field conditions. To promote breeding of disease resistant cultivar, it is important to investigate plant-fungi interaction under field conditions. In this study, RNA-seq data were collected from melon plants, and a bioinformatics method was considered to simultaneously analyze fungi composition and plant transcriptome based on a single RNA-seq data.

Academic Significance and Societal Importance of the Research Achievements

ウリ科植物には、メロンの他にカボチャ、キュウリなど農業生産上重要な園芸作物が存在する。いずれもうどんこ等の病原菌への抵抗性が望まれる作物であり、本研究で考案したRNA-seqに基づく一斉解析の手法はこれらのウリ科作物の研究においても応用が可能である。今後、より大規模なRNA-seqデータを圃場環境で収集した際には、菌類のプロファイルとトランスクリプトーム情報の統合解析が実施できると考えている。このような試みで得られた遺伝子解析情報は、将来の病害抵抗性品種の育成や重要遺伝子の発見のために役立つことが期待される。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (7 results)

All 2019 2018 2017 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (4 results) (of which Int'l Joint Research: 1 results) Remarks (2 results)

  • [Journal Article] Melonet-DB, a Grand RNA-Seq Gene Expression Atlas in Melon (Cucumis melo L.)2017

    • Author(s)
      Yano Ryoichi、Nonaka Satoko、Ezura Hiroshi
    • Journal Title

      Plant and Cell Physiology

      Volume: 59 Issue: 1 Pages: e4-e4

    • DOI

      10.1093/pcp/pcx193

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] メロンのゲノム解析およびデータベース構築2019

    • Author(s)
      矢野 亮一
    • Organizer
      園芸学会(2019年春季)ウリ科小集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Developing Genome and Transcriptome Information Database for Functional Genomics Research of Japanese Muskmelon2018

    • Author(s)
      Ryoichi Yano, Tohru Ariizumi, Satoko Nonaka, Hiroshi Ezura
    • Organizer
      Cucurbitaceae2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Pacbioを用いたマスクメロンの全ゲノム情報解読2018

    • Author(s)
      矢野 亮一
    • Organizer
      第134回 日本育種学会 ランチョンセミナー
    • Related Report
      2018 Annual Research Report
  • [Presentation] メロンのゲノム情報解析データベース「Melonet-DB」2018

    • Author(s)
      矢野亮一、野中聡子、江面浩
    • Organizer
      第59回日本植物生理学会
    • Related Report
      2017 Research-status Report
  • [Remarks] Melonet-DB

    • URL

      https://gene.melonet-db.jp/ap/top

    • Related Report
      2018 Annual Research Report
  • [Remarks] Melonet-DB

    • URL

      http://melonet-db.agbi.tsukuba.ac.jp/

    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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